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Titolo:
Species identification of formed fishery products and high pressure-treated fish by electrophoresis: a collaborative study
Autore:
Etienne, M; Jerome, M; Fleurence, J; Rehbein, H; Kundiger, R; Mendes, R; Costa, H; Martinez, I;
Indirizzi:
Inst Francais Rech Exploitat Mer, Lab BPQ VP, F-44311 Nantes 03, France Inst Francais Rech Exploitat Mer Nantes France 03 4311 Nantes 03, France Bundesforsch Anstalt Fischerei, Inst Biochem & Technol, D-22767 Hamburg, Germany Bundesforsch Anstalt Fischerei Hamburg Germany D-22767 Hamburg, Germany Inst Invest Pescas & Mar IPIMAR, P-1449006 Lisbon, Portugal Inst Invest Pescas & Mar IPIMAR Lisbon Portugal P-1449006 sbon, Portugal Norwegian Inst Fisheries & Aquaculture Ltd, N-9291 Tromso, Norway Norwegian Inst Fisheries & Aquaculture Ltd Tromso Norway N-9291 , Norway
Titolo Testata:
FOOD CHEMISTRY
fascicolo: 1, volume: 72, anno: 2001,
pagine: 105 - 112
SICI:
0308-8146(200101)72:1<105:SIOFFP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYACRYLAMIDE-GEL-ELECTROPHORESIS; SEAFOOD PRODUCTS; RAW;
Keywords:
electrophoresis; urea IEF; SDS-PAGE; identification; formed fish; high pressure; protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Etienne, M Inst Francais Rech Exploitat Mer, Lab BPQ VP, BP 21105, F-44311Nantes 03,France Inst Francais Rech Exploitat Mer BP 21105 Nantes France 03 nce
Citazione:
M. Etienne et al., "Species identification of formed fishery products and high pressure-treated fish by electrophoresis: a collaborative study", FOOD CHEM, 72(1), 2001, pp. 105-112

Abstract

The suitability and reliability of three electrophoretic methods of fish species identification, urea isoelectric focusing (IEF), sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) and native IEF, were evaluated on formed fish fillets and high pressure fish flesh by a collaborative study among four institutes. By following optimized standard operation procedures, the protein patterns of processed fish were compared to patternsof raw reference samples. The method to use depended of the effect of processing on the protein pattern. The proteins obtained from formed products were not denatured and therefore any of the three methods proved to be adequate, with a preference for native IEF which had a better discriminatory power for the species used. The high pressure process altered the proteins, and so only urea IEF and SDS-PAGE methods could be used. For these products, the chosen method should then be the one with the better discriminating power for the species being examined. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:28:19