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Titolo:
Biopharmaceutics of liposomal interleukin 2, Oncolipin
Autore:
Neville, ME; Boni, LT; Pflug, LE; Popescu, MC; Robb, RJ;
Indirizzi:
Biomirma USA Inc, Cranbury, NJ 08831 USA Biomirma USA Inc Cranbury NJ USA08831 ma USA Inc, Cranbury, NJ 08831 USA
Titolo Testata:
CYTOKINE
fascicolo: 11, volume: 12, anno: 2000,
pagine: 1691 - 1701
SICI:
1043-4666(200011)12:11<1691:BOLI2O>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT INTERLEUKIN-2; SUBCUTANEOUS INJECTION; LYMPHATIC UPTAKE; IL-2 RECEPTOR; BIODISTRIBUTION; IMMUNOASSAYS; BILAYERS; EUROPIUM; DELIVERY; BINDING;
Keywords:
IL-2; liposomes; receptors; internalization; release;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Neville, ME Biomirma USA Inc, 1002 Eastpk Blvd, Cranbury, NJ 08831 USA Biomirma USA Inc 1002 Eastpk Blvd Cranbury NJ USA 08831 31 USA
Citazione:
M.E. Neville et al., "Biopharmaceutics of liposomal interleukin 2, Oncolipin", CYTOKINE, 12(11), 2000, pp. 1691-1701

Abstract

Oncolipin is a multilamellar liposomal (dimyristoyl phosphatidylcholine) formulation of interleukin 2 (IL-2) and human serum albumin (HSA) with distinct surface characteristics which may influence its biological activities. IL-2 and HSA were detected on the surface of the liposomes using specific antibody staining. Surface expression of IL-2 was also demonstrated by the observation that Oncolipin bound to cells expressing IL-2 receptors (IL-2R) containing alpha beta gamma or beta gamma subunits, Binding and internalization of Oncolipin by cells expressing alpha beta gamma or beta gamma receptor subunits was blocked by excess free IL-2 or a neutralizing antibody against the beta chain. The display of surface IL-2 on Oncolipin's liposomes was maintained in vivo after intravenous injection into mice. IL-2 was also present between the lipid bilayers of the multilamellar liposomes based on the unique physical characteristics detected by freeze fracture electron microscopy, The bulk of the liposome-associated IL-2 was released from the liposomes upon incubation at 37 degreesC in medium containing serum, indicating that the IL-2 was not irreversibly entrapped on or in the liposome structure. Thus, Oncolipin is receptor-targeted to activated T and NK cells by virtue of its surface expression of IL-2 and has the potential to release IL-2 following deposition within lymphoid organs. These properties may confer distinct advantages over soluble IL-2 for immunotherapy of cancer and viraldiseases. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:15:49