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Titolo:
Role of cytokine-induced neutrophil chemoattractant-2 (cinc-2) alpha in a rat model of chronic bronchopulmonary infections with Pseudomonas aeruginosa
Autore:
Amano, H; Oishi, K; Sonoda, F; Senba, M; Wada, A; Nakagawa, H; Nagatake, T;
Indirizzi:
Nagasaki Univ, Dept Internal Med, Inst Trop Med, Nagasaki 8528523, Japan Nagasaki Univ Nagasaki Japan 8528523 t Trop Med, Nagasaki 8528523, Japan Toyama Med & Pharmaceut Univ, Fac Pharmaceut Sci, Dept Physiol Chem, Sugitani, Toyama 93001, Japan Toyama Med & Pharmaceut Univ Sugitani Toyama Japan 93001 ama 93001, Japan
Titolo Testata:
CYTOKINE
fascicolo: 11, volume: 12, anno: 2000,
pagine: 1662 - 1668
SICI:
1043-4666(200011)12:11<1662:ROCNC(>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE; GRO/CINC-2-ALPHA; INFLAMMATION; CHEMOKINES; CULTURE; GRO;
Keywords:
bronchopulmonary infections; CINC-2 alpha; neutrophil chemotactic factor; Pseudomonas aeruginosa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Amano, H Nagasaki Univ, Dept Internal Med, Inst Trop Med, 1-12-4 Sakamoto,Nagasaki8528523, Japan Nagasaki Univ 1-12-4 Sakamoto Nagasaki Japan 8528523 8523, Japan
Citazione:
H. Amano et al., "Role of cytokine-induced neutrophil chemoattractant-2 (cinc-2) alpha in a rat model of chronic bronchopulmonary infections with Pseudomonas aeruginosa", CYTOKINE, 12(11), 2000, pp. 1662-1668

Abstract

In order to investigate the role of the cytokine-induced neutrophil chemoattractant (CINC) in chronic bronchopulmonary infection, we developed a rat model of bronchopulmonary infection with Pseudomonas aeruginosa by using the agar bead method, and determined the kinetics of bacterial and cell number, as well as the concentrations of CINC-1, CINC-2, and CINC-3 in bronchoalveolar lavage (BAL) fluids in this model. The bacterial number in the lung rapidly increased from days 1 to 4, and declined 14 days after challenge. Neutrophil number in BAL fluid increased up to one day after challenge, and then slowly decreased during 14 days post-challenge, Among the CINCs, the local production of CINC3 alpha sharply increased at day 1 and then decreased until day 4 post-challenge, while the local production of CINC-1 slightlyincreased at day 1 post-challenge. Neither CINC-2 beta nor CINC-3 were detected during the entire course of the infection. Increased CINC-2 mRNA expression in the lung tissue after challenge was associated with CLNC-2 alpha production in BAL fluid, Moreover, an immunohistochemical study demonstrated the localization of CINC-1 and CINC-2 alpha primarily in alveolar macrophages and, to a much lesser extent, in bronchial epithelium of infected lungtissues, whereas CINC-2 beta and CINC-3 were not detected. When anti-CINC-1 or anti-CINC-2 alpha polyclonal antibodies were used for neutralizing neutrophil chemotactic activities in BAL fluids, the anti-CINC-2 alpha antibody inhibited 70% of the chemotactic activity in BAL fluids from infected rats at day 1 after challenge. No inhibition was observed by anti-CINC-1 antibody. These data indicate that CINC-2 alpha, which is produced by alveolar macrophages and bronchial epithelial cells, plays a pivotal role in neutrophil accumulation in the airway of a rat model of chronic bronchopulmonary infection with P. aeruginosa. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 08:33:17