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Titolo:
A single endotoxin injection in the rabbit causes prolonged blood vessel dysfunction and a procoagulant state
Autore:
Leclerc, J; Pu, Q; Corseaux, D; Haddad, E; Decoene, C; Bordet, R; Six, I; Jude, B; Vallet, B;
Indirizzi:
Univ Hosp Lille, Dept Anesthesiol & Intens Care, Lille, France Univ Hosp Lille Lille France t Anesthesiol & Intens Care, Lille, France Univ Hosp Lille, Dept Pharmacol, Lille, France Univ Hosp Lille Lille France Hosp Lille, Dept Pharmacol, Lille, France Univ Hosp Lille, Dept Cardiol, Lille, France Univ Hosp Lille Lille France iv Hosp Lille, Dept Cardiol, Lille, France Univ Hosp Lille, Dept Hematol, Lille, France Univ Hosp Lille Lille France iv Hosp Lille, Dept Hematol, Lille, France Fac Hlth Sci, Lille, France Fac Hlth Sci Lille FranceFac Hlth Sci, Lille, France
Titolo Testata:
CRITICAL CARE MEDICINE
fascicolo: 11, volume: 28, anno: 2000,
pagine: 3672 - 3678
SICI:
0090-3493(200011)28:11<3672:ASEIIT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
AORTIC ENDOTHELIUM; L-ARGININE; TISSUE FACTOR; INHIBITION; SEPSIS; INJURY; PATHWAY; SHOCK; MODEL; RATS;
Keywords:
endothelial cell; endothelium; sepsis; septic shock; endotoxin; lipopolysaccharide; rabbit; vascular reactivity; tissue factor; coagulation activation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Vallet, B Ctr Hosp Reg & Univ Lille, Hop Claude Huriez, Dept Anesthesie & Reanimat, 1 Pl Verdun, F-59037 Lille, France Ctr Hosp Reg & Univ Lille 1 PlVerdun Lille France F-59037 ance
Citazione:
J. Leclerc et al., "A single endotoxin injection in the rabbit causes prolonged blood vessel dysfunction and a procoagulant state", CRIT CARE M, 28(11), 2000, pp. 3672-3678

Abstract

Objectives: To determine the duration of vascular blood vessel dysfunctionand coagulation abnormalities after administration of endotoxin in a nonlethal septic rabbit model. Design: Randomized, controlled, interventional trial. Setting: University animal laboratory. Subjects: A total of 30 male New Zealand White rabbits, randomly assigned to one of two groups. Interventions: Male New Zealand White rabbits were randomly divided into control or lipopolysaccharide (LPS) (0.5 mg/kg iv bolus Escherichia coil endotoxin)-treated groups. Metabolic acidosis and coagulation activation confirmed the presence of septic shock. The abdominal aorta was removed at 24 hrs (day 1), day 5, or day 21 after LPS injection. Immunohistochemical staining for an endothelial cell marker (PECAM-1/CD31) was performed to assess endothelial injury. Endothelium-dependent vascular relaxation was analyzed byin vitro vascular reactivity studies. Responses to acetylcholine, to calcium ionophore (A-23187), and to sodium nitroprusside were studied. In addition, arterial blood samples were collected on day 1, day 5, and day 21 for measurement of clotting factors and tissue factor activity. Measurements and Main Results: LPS injection resulted in endothelial injury, with loss of similar to 25% of the endothelial area on day 5, which disappeared on day 21. LPS injection also caused a significantly reduced relaxation response to acetylcholine (44.9% +/-9.9% vs. 76.5% +/-5.4% for the control group; p < .005), which was restored on day 21. In contrast, vascular relaxation in response to A-23187 and sodium nitroprusside was not altered. A significant decrease in the platelet count was observed on day 1, associated with a decrease in factors II and V. On day 5, platelet count and factors II and V were corrected in conjunction with an elevated monocyte tissuefactor activity in LPS-injected rabbits. On day 21, coagulation abnormalities were corrected. Conclusions: A single endotoxin injection in the rabbit was responsible for prolonged aortic endothelial cell dysfunction, as well as a prolonged procoagulant state. The latter is a potential trigger for disseminated intravascular coagulation. Importantly, these features are associated with normalization of conventional biological evidence of septic shock.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 16:25:08