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Titolo:
Effect of embryo orientation on the developmental sequence of adventitiousorganogenesis in jack pine (Pinus banksiana Lamb.)
Autore:
Pelletier, G; Laliberte, S;
Indirizzi:
Univ Quebec, Dept Sci Biol, Grp Rech Ecol Forestiere Interuniv, Montreal, PQ H3C 3P8, Canada Univ Quebec Montreal PQ Canada H3C 3P8 univ, Montreal, PQ H3C 3P8, Canada
Titolo Testata:
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
fascicolo: 10, volume: 78, anno: 2000,
pagine: 1348 - 1360
SICI:
0008-4026(200010)78:10<1348:EOEOOT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
STROBUS COTYLEDONS INVITRO; MATURE EMBRYOS; REGENERATION; COMPETENCE; MEGAGAMETOPHYTE; CAULOGENESIS; GERMINATION; PLANTLETS; RADIATA; GROWTH;
Keywords:
adventitious organogenesis; embryo culture; explant orientation; jack pine; microscopy; Pinus banksiana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Laliberte, S Univ Quebec, Dept Sci Biol, Grp Rech Ecol Forestiere Interuniv, CP 8888,Succursale Ctr Ville, Montreal, PQ H3C 3P8, Canada Univ Quebec CP 8888,Succursale Ctr Ville Montreal PQ Canada H3C 3P8
Citazione:
G. Pelletier e S. Laliberte, "Effect of embryo orientation on the developmental sequence of adventitiousorganogenesis in jack pine (Pinus banksiana Lamb.)", CAN J BOTAN, 78(10), 2000, pp. 1348-1360

Abstract

The developmental sequence of adventitious organogenesis in jack pine (Pinus banksiana Lamb.) embryos was analysed depending on two explant orientations. Embryos were placed vertically (cotyledons downward) or horizontally on half-strength Schenk and Hildebrandt medium containing 10 muM 6-benzylaminopurine. Samples were harvested at 2- to 3-day intervals for a 21-day period and observed using light and electron microscopy. In vertically placed embryos, cotyledons were inserted into the medium and became necrotic, whilebuds and phylloids developed on the upper part of the hypocotyl and in theapical area, 14 and 9 days after culture initiation respectively. In horizontally cultured embryos, organogenesis started in the intercotyledonary areas (day 11) and spread to the abaxial surface of cotyledonary margins outside the medium (day 14). A few buds also developed on the adaxial surface of cotyledons, starting on day 16. In the apical area, phylloid formation started on day 18. Nodules were induced in tissues in contact with the mediumfor both orientations, but in distinct sites. Embryo orientation influenced the localization of induction sites and the time sequence of organogenesis. The pattern for the mobilization of proteins, lipids, and polysaccharides prior to organogenesis was similar for both orientations. A depletion in proteins and polysaccharides did not occur in tissues in contact with the culture medium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 10:28:42