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Titolo:
Ethanol stimulates glucose uptake and translocation of GLUT-4 in H9c2 myotubes via a Ca2+-dependent mechanism
Autore:
Yu, B; Schroeder, A; Nagy, LE;
Indirizzi:
Case Western Reserve Univ, Dept Nutr, Cleveland, OH 44106 USA Case WesternReserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 6, volume: 279, anno: 2000,
pagine: E1358 - E1365
SICI:
0193-1849(200012)279:6<E1358:ESGUAT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKELETAL-MUSCLE; VESICULAR TRANSPORT; INSULIN-SECRETION; CALCIUM CHANNELS; CELLS; RAT; RECEPTOR; VESICLES; ENDOCYTOSIS; EXPRESSION;
Keywords:
skeletal muscle; glucose tolerance; insulin; phosphatidylinositol 3-kinase; vesicle trafficking;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Nagy, LE Case Western Reserve Univ, Dept Nutr, 2123 Abington Rd,Rm 201, Cleveland, OH 44106 USA Case Western Reserve Univ 2123 Abington Rd,Rm 201 Cleveland OH USA 44106
Citazione:
B. Yu et al., "Ethanol stimulates glucose uptake and translocation of GLUT-4 in H9c2 myotubes via a Ca2+-dependent mechanism", AM J P-ENDO, 279(6), 2000, pp. E1358-E1365

Abstract

Short-term exposure to ethanol impairs glucose homeostasis, but the effects of ethanol on individual components of the glucose disposal pathway are not known. To understand the mechanisms by which ethanol disrupts glucose homeostasis, we have investigated the direct effects of ethanol on glucose uptake and translocation of GLUT-4 in H9c2 myotubes. Short-term treatment with 12.5-50 mM ethanol increased uptake of 2-deoxyglucose by 1.8-fold in differentiated myotubes. Pretreatment of H9c2 myotubes with 100 nM wortmannin, an inhibitor of phosphatidylinositol 3-kinase, had no effect on ethanol-induced increases in 2-deoxyglucose uptake. In contrast, preincubation with 25mM dantrolene, an inhibitor of Ca2+ release from the sarcoplasmic reticulum, blocked the stimulation of 2-deoxyglucose uptake by ethanol. Increased 2-deoxyglucose uptake after ethanol treatment was associated with a decreasein small intracellular GLUT-4 vesicles and an increase in GLUT-4 localizedat the cell surface. In contrast, ethanol had no effect on the quantity ofGLUT-1 and GLUT-3 at the plasma membrane. These data demonstrate that physiologically relevant concentrations of ethanol disrupt the trafficking of GLUT-4 in H9c2 myotubes resulting in translocation of GLUT-4 to the plasma membrane and increased glucose uptake.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:04:36