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Titolo:
Sodium-hydrogen exchange in human platelets exposed to weak acid: comparison between measurements of platelet swelling and cytoplasmic sodium ion concentration
Autore:
Stratton, PD; Chowienczyk, PJ; Ritter, JM;
Indirizzi:
Univ London Kings Coll, St Thomas Hosp, Dept Clin Pharmacol, GKT Sch Biomed Sci, London SE1 7EH, England Univ London Kings Coll London England SE1 7EH i, London SE1 7EH, England
Titolo Testata:
PLATELETS
fascicolo: 7, volume: 11, anno: 2000,
pagine: 401 - 405
SICI:
0953-7104(200011)11:7<401:SEIHPE>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NA+-H+ EXCHANGE; ESSENTIAL-HYPERTENSION; PROTON EXCHANGE; ANTIPORT ACTIVITY; INTRACELLULAR PH; INSULIN; NEPHROPATHY; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ritter, JM Univ London Kings Coll, St Thomas Hosp, Dept Clin Pharmacol, GKT Sch Biomed Sci, Lambeth Palace Rd, London SE1 7EH, England Univ London Kings Coll Lambeth Palace Rd London England SE1 7EH
Citazione:
P.D. Stratton et al., "Sodium-hydrogen exchange in human platelets exposed to weak acid: comparison between measurements of platelet swelling and cytoplasmic sodium ion concentration", PLATELETS, 11(7), 2000, pp. 401-405

Abstract

Increased Na+-H+ exchange activity has been demonstrated in various pathological states using the rate constant of platelet swelling (k(s)). This indirect measure of exchange activity could, however, be influenced by conditions that alter platelet distensibility independent of Na+-H+ exchange activity. To determine the validity of k(s), the change in light transmission and hence k(s), was measured at 24 degrees and 37 degreesC (temperatures associated with alterations in membrane fluidity), and compared with the rate constant (k(Na)) for the increase in cytoplasmic sodium ion concentration ([Na+](i)) and the rate constant of Na+-influx (k(phi Na)) under the same conditions. Both k(s) and k(Na) were dependent on Na+-H+ exchange; the rate constants differed in the order k(s) < k(<phi>Na) < k(Na) (P<0.01). Neither k(Na) or k(phi Na) changed with temperature, whereas k(s) was significantly lower at 24 degrees than at 37 degreesC (7.49 +/- 0.31x10(-3) s(-1) versus 9.96 +/- 0.42x10(-3) s(-1), P<0.0001). While both k(s) and k(Na) are dependent on Na+-H+ exchange, k(s) may underestimate the activity of the exchanger and may in part, be influenced by membrane fluidity. This could be important in interpreting data where k(s) has been measured in different groups of subjects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:23:38