Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Chitinase genes responsive to cold encode antifreeze proteins in winter cereals
Autore:
Yeh, S; Moffatt, BA; Griffith, M; Xiong, F; Yang, DSC; Wiseman, SB; Sarhan, F; Danyluk, J; Xue, YQ; Hew, CL; Doherty-Kirby, A; Lajoie, G;
Indirizzi:
Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 ol, Waterloo, ON N2L 3G1, Canada Univ Toronto, Deppt Lab Med & Pathobiol, Toronto, ON, Canada Univ TorontoToronto ON Canada Lab Med & Pathobiol, Toronto, ON, Canada McMaster Univ, Dept Biochem, Hamilton, ON L8N 3Z5, Canada McMaster Univ Hamilton ON Canada L8N 3Z5 em, Hamilton, ON L8N 3Z5, Canada Univ Quebec, Dept Sci Biol, Montreal, PQ H3C 3P8, Canada Univ Quebec Montreal PQ Canada H3C 3P8 Biol, Montreal, PQ H3C 3P8, Canada Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 em, Waterloo, ON N2L 3G1, Canada
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 3, volume: 124, anno: 2000,
pagine: 1251 - 1263
SICI:
0032-0889(200011)124:3<1251:CGRTCE>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROXYPROLINE-CONTAINING PROTEINS; PATHOGENESIS-RELATED PROTEINS; THERMAL HYSTERESIS PROTEINS; RICH-REPEAT PROTEIN; LOW-TEMPERATURE; FREEZING TOLERANCE; PLANT CHITINASES; RYE LEAVES; BINDING-PROTEINS; CELL-SHAPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Griffith, M Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 , ON N2L 3G1, Canada
Citazione:
S. Yeh et al., "Chitinase genes responsive to cold encode antifreeze proteins in winter cereals", PLANT PHYSL, 124(3), 2000, pp. 1251-1263

Abstract

Antifreeze proteins similar to two different chitinases accumulate during cold acclimation in winter rye (Secale cereale). To determine whether thesecold-responsive chitinases require post-translational modification to bindto ice, cDNAs coding for two different full-length chitinases were isolated from a cDNA library produced from cold-acclimated winter rye leaves. CHT9is a 1,193-bp clone that encodes a 31.7-kD class I chitinase and CHT46 is a 998-bp clone that codes for a 24.8-kD class II chitinase. Chitinase-antifreeze proteins purified from the plant were similar in mass to the predicted mature products of CHT9 and CHT46, thus indicating that there was little chemical modification of the amino acid sequences in planta. To confirm these results, the mature sequences of CHT9 and CHT46 were expressed in Escherichia coli and the products of both cDNAs modified the growth of ice. Transcripts of both genes accumulated late in cold acclimation in winter rye. Southern analysis of winter rye genomic DNA indicated the presence of a smallgene family homologous to CHT46. In hexaploid wheat, CHT46 homologs marredto the homeologous group 1 chromosomes and were expressed in response to cold and drought. We conclude that two novel cold-responsive genes encoding chitinases with ice-binding activity may have arisen in winter rye and other cereals through gene duplication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:17:23