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Titolo:
Error-free and error-prone lesion bypass by human DNA polymerase kappa in vitro
Autore:
Zhang, YB; Yuan, FH; Wu, XH; Wang, M; Rechkoblit, O; Taylor, JS; Geacintov, NE; Wang, ZG;
Indirizzi:
Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 Ctr Toxicol, Lexington, KY 40536 USA Washington Univ, Dept Chem, St Louis, MO 63130 USA Washington Univ St Louis MO USA 63130 , Dept Chem, St Louis, MO 63130 USA NYU, Dept Chem, New York, NY 10003 USA NYU New York NY USA 10003NYU, Dept Chem, New York, NY 10003 USA
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 21, volume: 28, anno: 2000,
pagine: 4138 - 4146
SICI:
0305-1048(20001101)28:21<4138:EAELBB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI DINB; SACCHAROMYCES-CEREVISIAE GENE; APURINIC APYRIMIDINIC SITE; THYMINE-THYMINE DIMER; MAMMALIAN-CELLS; XERODERMA-PIGMENTOSUM; INDUCED MUTAGENESIS; ABASIC SITES; SEQUENCE CONTEXT; MOUSE HOMOLOGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, ZG Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ol, Lexington, KY 40536 USA
Citazione:
Y.B. Zhang et al., "Error-free and error-prone lesion bypass by human DNA polymerase kappa in vitro", NUCL ACID R, 28(21), 2000, pp. 4138-4146

Abstract

Error-free lesion bypass and error-prone lesion bypass are important cellular responses to DNA damage during replication, both of which require a DNApolymerase (Pol). To identify lesion bypass DNA polymerases, we have purified human Pol kappa encoded by the DINB1 gene and examined its response to damaged DNA templates. Here, we show that Human Pol kappa is a novel lesionbypass polymerase in vitro. Purified human Pol kappa efficiently bypassed a template 8-oxoguanine, incorporating mainly A and less frequently C opposite the lesion. Human Pol kappa most frequently incorporated A opposite a template abasic site. Efficient further extension required T as the next template base, and was mediated mainly by a one-nucleotide deletion mechanism. Human Pol kappa was able to bypass an acetylaminofluorene-modified G in DNA, incorporating either C or T, and less efficiently A opposite the lesion. Furthermore, human Pol kappa effectively bypassed a template (-)-trans-antibenzo[a]pyrene-N-2-dG lesion in an error-free manner by-incorporating a C opposite the bulky adduct. In contrast, human Pol kappa was unable to bypass a template TT dimer or a TT (6-4) photoproduct, two of the major UV lesions. These results suggest that Poi kappa plays an important role in both error-free and error-prone lesion bypass in humans.

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Documento generato il 03/06/20 alle ore 09:38:02