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Titolo:
Phosphofructokinase, a glycolytic regulatory enzyme has a crucial role formaintenance of synaptic activity in guinea pig hippocampal slices
Autore:
Li, XX; Yokono, K; Okada, Y;
Indirizzi:
Kobe Univ, Sch Med, Dept Physiol, Chuo Ku, Kobe, Hyogo 6500017, Japan KobeUniv Kobe Hyogo Japan 6500017 l, Chuo Ku, Kobe, Hyogo 6500017, Japan
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 2, volume: 294, anno: 2000,
pagine: 81 - 84
SICI:
0304-3940(20001117)294:2<81:PAGREH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURAL ACTIVITY; GLUCOSE-METABOLISM; PRESERVATION; FRUCTOSE; MANNOSE; LACTATE; BRAIN;
Keywords:
hippocampal slices; synaptic activity; high energy phosphates; anaerobic glycolysis; phosphofructokinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Okada, Y Kobe Univ, Sch Med, Dept Physiol, Chuo Ku, 7-5-1 Kusunoki Cho, Kobe, Hyogo6500017, Japan Kobe Univ 7-5-1 Kusunoki Cho Kobe Hyogo Japan 6500017 0017, Japan
Citazione:
X.X. Li et al., "Phosphofructokinase, a glycolytic regulatory enzyme has a crucial role formaintenance of synaptic activity in guinea pig hippocampal slices", NEUROSCI L, 294(2), 2000, pp. 81-84

Abstract

To clarify the importance of glucose metabolism in maintaining neural activity, we investigated the effects of lowering the concentration of glucose in perfusion medium on synaptic activity (population spikes, PS) and on thelevel of high energy phosphates in the region of dentate gyrus (DG), CA3 and CA1 area of hippocampal slices of guinea pig. Further we determined the activity of phosphofructokinase (PFK) and hexokinase (HK) of these areas. Lowering the concentration of glucose from 10 to 5, 4, 3, 1 and 0 (mM) caused a reduction in the PS amplitude. The PS in CA3 and CA1 region decayed andextinguished much faster than that in DG area. The ATP and CrP levels in these three areas were well preserved even after lowering the concentration of glucose to 3 mM, and the patterns of reduction showed no difference in these three areas. On the other hand, there were significant differences in the activity of PFK in the DG, CA3 and CA1 areas, in contrast with HK whichshowed no significant differences. PFK activity was highest in the DG areaand followed by CA3 and CA1 regions. The sensitivity in maintaining synaptic activity to lowered glucose concentrations showed good parallels with the activity of PFK in these regions, indicating that non-oxidative glycolytic metabolism regulated by PFK is crucial for the maintenance of synaptic activity (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:49:26