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Titolo:
The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a Streptomyces enzyme
Autore:
Rivas, LA; Parro, V; Moreno-Paz, M; Mellado, RP;
Indirizzi:
Univ Autonoma Madrid, CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain UnivAutonoma Madrid Madrid Spain E-28049 otecnol, E-28049 Madrid, Spain
Titolo Testata:
MICROBIOLOGY-UK
, volume: 146, anno: 2000,
parte:, 11
pagine: 2929 - 2936
SICI:
1350-0872(200011)146:<2929:TBS1CG>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION INITIATION SITES; BACTERIOPHAGE PHI-29 DNA; INVIVO TRANSCRIPTION; PROMOTER SEQUENCES; ESCHERICHIA-COLI; PURIFICATION; PROTEIN; AGARASE; N174; OVERPRODUCTION;
Keywords:
chitosanase; Bacillus subtilis; gene expression; catabolite repression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Mellado, RP Univ Autonoma Madrid, CSIC, Ctr Nacl Biotecnol, Campus,Cantoblanco, E-28049 Madrid, Spain Univ Autonoma Madrid Campus,Cantoblanco MadridSpain E-28049
Citazione:
L.A. Rivas et al., "The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a Streptomyces enzyme", MICROBIO-UK, 146, 2000, pp. 2929-2936

Abstract

The Bacillus subtilis 168 csn gene encodes a chitosanase. It was found that transcription of the csn gene was temporally regulated and was not subject to metabolic repression. Chitosanase synthesis was abolished in a csn mutant strain. Csn was overproduced in B. subtilis, partially purified and characterized. The deduced amino acid sequence, K-m, and optimal pH and temperature of the B. subtilis enzyme were closer to those of a chitosanase from Streptomyces sp. N174 than to those of chitosanases from other Bacillus strains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:20:14