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Titolo:
Quantitative autoradiographic mapping of rat brain dopamine D3 binding with [I-125]7-OH-PIPAT: Evidence for the presence of D3 receptors on dopaminergic and nondopaminergic cell bodies and terminals
Autore:
Stanwood, GD; Artymyshyn, RP; Kung, MP; Kung, HF; Lucki, I; McGonigle, P;
Indirizzi:
Univ Penn, Sch Med, Dept Pharmacol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Pharmacol, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Dept Radiol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 pt Radiol, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Dept Psychiat, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Psychiat, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Inst Neurol Sci, Philadelphia, PA 19104 USA Univ PennPhiladelphia PA USA 19104 eurol Sci, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
fascicolo: 3, volume: 295, anno: 2000,
pagine: 1223 - 1231
SICI:
0022-3565(200012)295:3<1223:QAMORB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
D-3 RECEPTOR; NUCLEUS-ACCUMBENS; PARADOXICAL REGULATION; INSITU HYBRIDIZATION; STRIATAL DOPAMINE; MESSENGER-RNA; NEURONS; LOCALIZATION; EXPRESSION; SUBTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: McGonigle, P Wyeth Ayerst Res, Neuropsychiat Disorder Res, Div Neurosci, CN-8000, Princeton, NJ 08543 USA Wyeth Ayerst Res CN-8000 Princeton NJ USA 08543 NJ 08543 USA
Citazione:
G.D. Stanwood et al., "Quantitative autoradiographic mapping of rat brain dopamine D3 binding with [I-125]7-OH-PIPAT: Evidence for the presence of D3 receptors on dopaminergic and nondopaminergic cell bodies and terminals", J PHARM EXP, 295(3), 2000, pp. 1223-1231

Abstract

The regional distribution and cellular localization of dopamine D3 receptors in the rat brain was examined using quantitative autoradiography. [I-125]7-OH-PIPAT bound in a saturable and reversible manner and exhibited subnanomolar affinity for a single population of GTP-insensitive sites. The pharmacological profile was characteristic of cloned D3 receptors and nonspecific binding was uniformly low. The highest levels of D3 receptors were measured in the islands of Calleja, nucleus accumbens, ventral pallidum, substantia nigra, and lobules 9 and 10 of the cerebellum. The high specific activity of this ligand also allowed detection of D3 receptors in other regions, including the serotonergic dorsal and median raphe nuclei, indicating that the distribution of this receptor is more widespread than previously appreciated. The cellular localization of D3 receptors in regions containing dopaminergic cells and terminals was examined by discrete injection of neurotoxins. Lesion of dopaminergic neurons with 6-hydroxydopamine produced 50% decreases in [I-125]7-OH-PIPAT binding in the nucleus accumbens and substantia nigra. Quinolinic acid lesion of neurons originating in the nucleus accumbens also produced approximately 50% decreases in D3 receptors in the nucleusaccumbens, substantia nigra, and ventral pallidum. 5,7-Dihydroxytryptaminelesion of serotonergic cells and processes produced no changes in [I-125]7-OH-PIPAT binding. These results demonstrate the presence of D3 receptors in several brain regions not previously identified and suggest that D3 receptors are expressed at somatodendritic and terminal levels of both dopaminergic and nondopaminergic cells within the mesolimbic dopamine system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:20:13