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Titolo:
Degradation of glial glutamate transporter mRNAs is selectively blocked byinhibition of cellular transcription
Autore:
Zelenaia, OA; Robinson, MB;
Indirizzi:
Univ Penn, Childrens Hosp Philadelphia, Dept Pediat, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 pt Pediat, Philadelphia, PA 19104 USA Univ Penn, Childrens Hosp Philadelphia, Dept Pharmacol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Pharmacol, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 6, volume: 75, anno: 2000,
pagine: 2252 - 2258
SICI:
0022-3042(200012)75:6<2252:DOGGTM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA STABILITY; FIBROBLAST GROWTH-FACTOR; GENE-EXPRESSION; CYCLIC-AMP; DEPENDENT REGULATION; CORTICAL ASTROCYTES; PRIMARY CULTURES; BRAIN INJURY; RAT-BRAIN; PROTEINS;
Keywords:
glutamate aspartate transporter; glutamate transporter 1; glial glutamate transporters; transcription inhibition; astrocyte differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Robinson, MB 502N Abramson Pediat Res Bldg,3516 Civic Ctr Blvd, Philadelphia, PA 19104 USA 502N Abramson Pediat Res Bldg,3516 Civic Ctr Blvd Philadelphia PA USA 19104
Citazione:
O.A. Zelenaia e M.B. Robinson, "Degradation of glial glutamate transporter mRNAs is selectively blocked byinhibition of cellular transcription", J NEUROCHEM, 75(6), 2000, pp. 2252-2258

Abstract

Recent studies have demonstrated that the expression of the glial glutamate transporters GLT-1 (glutamate transporter 1) and GLAST (glutamate aspartate transporter) is regulated both in vivo and in vitro. For example, co-culturing with neurons, treatment with N-6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate (dbcAMP), and treatment with epidermal growth factor all increase the steady-state levels of GLT-1 and GLAST protein in astrocyte cultures. These changes in protein expression are correlated with increased mRNA levels. In the present study, the degradation of GLT-1 and GLAST mRNAs was examined in control and dbcAMP-treated astrocyte cultures after inhibiting transcription with actinomycin D, Although one would predict that inhibition of transcription would cause a decrease in GLT-1 and GLAST mRNAs and that this decrease would depend on the rate of mRNA degradation, the levels of GLT-1 and GLAST mRNAs did not decrease even after 24 h of treatment with actinomycin D. Withdrawal of dbcAMP caused the levels of GLT-1 and GLAST mRNAs to fall to basal levels within 24 h, but this degradation was blocked if actinomycin D was added at the time of dbcAMP withdrawal. Importantly, actinomycin D did not block the degradation of c-fos mRNA also induced by dbcAMP in these cultures. inhibition of translation with cycloheximide did notstabilize GLT-1 but partially attenuated the degradation of GLAST mRNA. Although the mechanism of this effect remains to be defined, these studies suggest that GLT-1 and GLAST mRNAs belong to a select class of inducible mRNAs stabilized by inhibitors of transcription. The possible relevance of these data to astrocyte differentiation is briefly discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:43:51