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Titolo:
Use of cryostat sections from snap-frozen nervous tissue for combining stereological estimates with histological, cellular, or molecular analyses on adjacent sections
Autore:
Schmitz, C; Dafotakis, M; Heinsen, H; Mugrauer, K; Niesel, A; Popken, GJ; Stephan, M; Van de Berg, WDJ; von Horsten, S; Korr, H;
Indirizzi:
Univ Aachen, RWTH, Dept Anat & Cell Biol, D-52057 Aachen, Germany Univ Aachen Aachen Germany D-52057 & Cell Biol, D-52057 Aachen, Germany Univ Wurzburg, Morphol Brain Res Unit, D-97080 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97080 s Unit, D-97080 Wurzburg, Germany Univ Calif Davis, Ctr Neurosci, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 is, Ctr Neurosci, Davis, CA 95616 USA Hannover Med Sch, Dept Funct & Appl Anat, D-30625 Hannover, Germany Hannover Med Sch Hannover Germany D-30625 nat, D-30625 Hannover, Germany Maastricht Univ, Dept Pediat Psychiat & Neuropsychol, NL-6212 AZ Maastricht, Netherlands Maastricht Univ Maastricht Netherlands NL-6212 AZ aastricht, Netherlands
Titolo Testata:
JOURNAL OF CHEMICAL NEUROANATOMY
fascicolo: 1, volume: 20, anno: 2000,
pagine: 21 - 29
SICI:
0891-0618(200010)20:1<21:UOCSFS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE HIPPOCAMPUS; PURKINJE-CELLS; OPTICAL FRACTIONATOR; CEREBELLAR CORTEX; SYSTEMATIC BIAS; NEURONAL LOSS; TOTAL NUMBER; AGED RATS; NUCLEUS; MEMORY;
Keywords:
cryostat sections; snap-frozen; stereology; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Schmitz, C Univ Aachen, RWTH, Dept Anat & Cell Biol, Pauwelsstr Wendlingweg 2, D-52057 Aachen, Germany Univ Aachen Pauwelsstr Wendlingweg 2 Aachen Germany D-52057 ny
Citazione:
C. Schmitz et al., "Use of cryostat sections from snap-frozen nervous tissue for combining stereological estimates with histological, cellular, or molecular analyses on adjacent sections", J CHEM NEUR, 20(1), 2000, pp. 21-29

Abstract

Adequate tissue preparation is essential for both modern stereological andimmunohistochemical investigations. However, combining these methodologiesin a single study presents a number of obstacles pertaining to optimal histological preparation. Tissue shrinkage and loss of nuclei/nucleoli from the unprotected section surfaces of unembedded tissue used for immunohistochemistry may be problematic with regard to adequate stereological design. In this study, frozen cryostat sections from hippocampal and cerebellar regions of two rat strains and cerebellar and cerebral regions from a human brainwere analyzed to determine the potential impact of these factors on estimates of neuron number obtained using the optical disector. Neuronal nuclei and nucleoli were clearly present in thin sections of snap-frozen rat (3 mum) and human (6 mum) tissue, indicating that neuronal nuclei/nucleoli are not unavoidably lost from unprotected section surfaces of unembedded tissue. In order to quantify the potential impact of any nuclear loss, optical fractionator estimates of rat hippocampal pyramidal cells in areas CA1-3 and cerebellar granule and Purkinje cells were made using minimal (1 mum) upper guard zones. Estimates did nut differ from data reported previously in the literature. This data indicates that cryostat sections of snap-frozen nervous tissue may successfully be used for estimating total neuronal numbers using optical disectors. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 08:42:23