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Titolo:
Antiviral resistance of biologic HIV-2 clones obtained from individuals onnucleoside reverse transcriptase inhibitor therapy
Autore:
van der Ende, ME; Guillon, C; Boers, PHM; Ly, TD; Gruters, RA; Osterhaus, ADME; Schutten, M;
Indirizzi:
Univ Hosp Ctr Rotterdam, Dept Internal Med, Rotterdam, Netherlands Univ Hosp Ctr Rotterdam Rotterdam Netherlands d, Rotterdam, Netherlands Univ Hosp Ctr Rotterdam, Dept Virol, Rotterdam, Netherlands Univ Hosp Ctr Rotterdam Rotterdam Netherlands l, Rotterdam, Netherlands Alfred Fournier Inst, Paris, France Alfred Fournier Inst Paris FranceAlfred Fournier Inst, Paris, France Ecole Normale Super Lyon, CNRS, F-69364 Lyon, France Ecole Normale Super Lyon Lyon France F-69364 CNRS, F-69364 Lyon, France
Titolo Testata:
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
fascicolo: 1, volume: 25, anno: 2000,
pagine: 11 - 18
SICI:
1525-4135(20000901)25:1<11:AROBHC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; COMBINATION THERAPY; ZIDOVUDINE MONOTHERAPY; CUBIC MILLIMETER; INFECTED ADULTS; LAMIVUDINE; DIDANOSINE; VARIANTS; DISEASE; PLASMA;
Keywords:
HIV-2; drug resistance/resistance mutations; antiretroviral therapy; nucleoside reverse transcriptase inhibitors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Osterhaus, ADME Erasmus Univ, Inst Virol, POB 1738, NL-3000 DR Rotterdam, Netherlands Erasmus Univ POB 1738 Rotterdam Netherlands NL-3000 DR ds
Citazione:
M.E. van der Ende et al., "Antiviral resistance of biologic HIV-2 clones obtained from individuals onnucleoside reverse transcriptase inhibitor therapy", J ACQ IMM D, 25(1), 2000, pp. 11-18

Abstract

Objective: To study phenotypic and genotypic resistance of HIV-2 against nucleoside reverse transcriptase inhibitors (NRTI). Methods: Biologic HIV-2 clones were generated from 3 patients before and after initiation of antiretroviral therapy with zidovudine (AZT) in patient RH2-7, AZT and didanosine (ddI) in patient PH2-1, and after addition of lamivudine (3TC) to AZT monotherapy in patient RH2-5. The sensitivity to NRTI of the virus clones, as defined by the 50% inhibitory concentration (IC50),was determined in vitro. The predicted amino acid sequences of the reversetranscriptase proteins from these clones were determined. Results: Comparing the sensitivity of the biologic HIV-2 clones obtained after start of therapy with those from antiviral naive patients, resistance had developed to AZT (patients RH2-7 and RH2-5) and 3TC (patient PH2-1 and RH2-5). No resistance to AZT was observed in the biologic clone from PH2-1 obtained after start of therapy. The resistant clones from RH2-5 and PH2-1,but not RH2-7, contained amino acid mutations at positions where HIV-1 hasbeen shown to mutate after AZT and 3TC treatment. Conclusions: Phenotypic resistance of HIV-2 to nucleoside analogues, whichdeveloped in HIV-2-infected patients treated with NRTIs, was associated with genotypic changes. Some of the mutations at amino acid positions in the HIV-2 reverse transcriptase gene corresponded with those involved in HIV-1 resistance, although no conventional mutations associated with resistance to AZT were observed.

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Documento generato il 31/03/20 alle ore 10:26:14