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Titolo:
Detection of autoreactive T cells in H-2(u) mice using peptide-MHC multimers
Autore:
Radu, CG; Anderton, SM; Firan, M; Wraith, DC; Ward, ES;
Indirizzi:
Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Bristol, Dept Pathol & Microbiol, Bristol BS8 1TD, Avon, England UnivBristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England
Titolo Testata:
INTERNATIONAL IMMUNOLOGY
fascicolo: 11, volume: 12, anno: 2000,
pagine: 1553 - 1560
SICI:
0953-8178(200011)12:11<1553:DOATCI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYELIN BASIC-PROTEIN; CLASS-II MHC; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; NONOBESE DIABETIC MICE; AUTOIMMUNE ENCEPHALOMYELITIS; QUANTITATIVE-ANALYSIS; IN-VIVO; TRANSGENIC MICE; ANTIGEN; RECEPTOR;
Keywords:
autoimmunity; clonal expansion; experimental autoimmune encephalomyelitis; MHC class II tetramers; T lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ward, ES Univ Texas, SW Med Ctr, Ctr Immunol, 5323 Harry Hines Blvd, Dallas, TX 75235 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 TX 75235 USA
Citazione:
C.G. Radu et al., "Detection of autoreactive T cells in H-2(u) mice using peptide-MHC multimers", INT IMMUNOL, 12(11), 2000, pp. 1553-1560

Abstract

Myelin basic protein (MBP)-specific T cells play a critical role in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), a prototype for T cell-mediated autoimmunity, In PL/J and B10.PL mice (H-2(U) haplotype), the immunodominant epitope of Map is represented by an N-terminal nonameric peptide, MBP1-9, To date, the MBP1-9-specific T cell repertoire has notbeen analyzed in quantitative terms, In the present study we demonstrate, using MHC class II tetramers, that 15,000-70,000 self-antigen-specific T-h cells accumulate in the draining lymph nodes following immunization with spinal cord homogenate or MBP1-9, In contrast, MBP1-9-specific T cells are undetectable in unimmunized H-2(U) mice and represent >60% of the CD4 cells in naive mice transgenic for a TCR specific for this epitope, The results suggest that the extremely low affinity of the N-terminal peptide for I-A(U) does not limit the MBP1-9-specific T cells from expanding into a sizeable pool of autoreactive T cells, Therefore, the primary immune response to MBP1-9 does not differ quantitatively from previously reported CD4(+) T cell responses to foreign antigens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:24:06