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Titolo:
Requirement for Atr in phosphorylation of Chk1 and cell cycle regulation in response to DNA replication blocks and UV-damaged DNA in Xenopus egg extracts
Autore:
Guo, ZJ; Kumagai, A; Wang, SX; Dunphy, WG;
Indirizzi:
CALTECH, Howard Hughes Med Inst, Div Biol, Pasadena, CA 91125 USA CALTECHPasadena CA USA 91125 Med Inst, Div Biol, Pasadena, CA 91125 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 21, volume: 14, anno: 2000,
pagine: 2745 - 2756
SICI:
0890-9369(20001101)14:21<2745:RFAIPO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE; IONIZING-RADIATION; CHECKPOINT CONTROL; SCHIZOSACCHAROMYCES-POMBE; ATAXIA-TELANGIECTASIA; CDC25 PHOSPHATASE; FUSION PROTEINS; S-PHASE; YEAST; GENE;
Keywords:
Atr; Chk1; phosphorylation; replication checkpoint; mitosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Dunphy, WG CALTECH, Howard Hughes Med Inst, Div Biol, 216-76, Pasadena, CA91125 USA CALTECH 216-76 Pasadena CA USA 91125 76, Pasadena, CA 91125 USA
Citazione:
Z.J. Guo et al., "Requirement for Atr in phosphorylation of Chk1 and cell cycle regulation in response to DNA replication blocks and UV-damaged DNA in Xenopus egg extracts", GENE DEV, 14(21), 2000, pp. 2745-2756

Abstract

The checkpoint kinase Xchk1 becomes phosphorylated in Xenopus egg extractsin response to DNA replication blocks or UV-damaged DNA. Xchk1 is also required for the cell cycle delay that is induced by unreplicated or UV-damaged DNA. In this report, we have removed the Xenopus homolog of ATR (Xatr) from egg extracts by immunodepletion. In Xatr-depleted extracts, the checkpoint-associated phosphorylation of Xchk1 is abolished, and the cell cycle delay induced by replication blocks is strongly compromised. Xatr from egg extracts phosphorylated recombinant Xchk1 in vitro, but not a mutant form of Xchk1 (Xchk1-4AQ) containing nonphosphorylatable residues in its four conserved SQ/TQ motifs. Recombinant human ATR, but not a kinase-inactive mutant, phosphorylated the same sites in Xchk1. Furthermore, the Xchk1-4AQ mutant was found to be defective in mediating a checkpoint response in egg extracts. These findings suggest that Xchk1 is a functionally important target of Xatr during a checkpoint response to unreplicated or UV-damaged DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 10:15:56