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Titolo:
Genomic analysis and functional expression of canine dopamine D2 receptor
Autore:
Myeong, H; Jeoung, DI; Kim, HK; Lee, YS; Kim, KH; Ha, JH; Park, C; Kaang, BK;
Indirizzi:
Korea Adv Inst Sci & Technol, Dept Biol Sci, Natl Creat Res Initiat Ctr Behav Genet, Taejon 305701, South Korea Korea Adv Inst Sci & Technol TaejonSouth Korea 305701 5701, South Korea Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Inst Mol Biol & Genet,Mol Neurobiol Lab, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 Lab, Seoul 151742, South Korea Kyungpook Natl Univ, Dept Gen Engn, Taegu 702701, South Korea Kyungpook Natl Univ Taegu South Korea 702701 , Taegu 702701, South Korea
Titolo Testata:
GENE
fascicolo: 1, volume: 257, anno: 2000,
pagine: 99 - 107
SICI:
0378-1119(20001017)257:1<99:GAAFEO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
D2-DOPAMINE RECEPTOR; SEQUENCE-ANALYSIS; G-PROTEINS; RAT; CLONING; CDNA; ISOFORMS; FORMS; BRAIN; IDENTIFICATION;
Keywords:
alternative splicing; G protein-activated inwardly rectifying K+ channel (GIRK); polymorphism; Xenopus oocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Park, C Korea Adv Inst Sci & Technol, Dept Biol Sci, Natl Creat Res Initiat Ctr Behav Genet, Taejon 305701, South Korea Korea Adv Inst Sci & TechnolTaejon South Korea 305701 uth Korea
Citazione:
H. Myeong et al., "Genomic analysis and functional expression of canine dopamine D2 receptor", GENE, 257(1), 2000, pp. 99-107

Abstract

Dopamine D2 receptor (DRD2) is one of the five dopamine receptors with seven transmembrane domains that are coupled to the G protein. We have cloned and characterized the genomic and cDNA sequences of the canine DRD2 gene, which are 12.7 and 2.7 kb in size, respectively. The genomic DNA is composedof seven exons and six introns, encoding a 443 amino acid protein with 95%amino acid identity to other mammalian D2 receptors. A length polymorphismwas detected in intron 3 of the receptor gene. We also characterized alternatively spliced forms of DRD2 cDNAs, DRD2L and DRD2S. They showed a higherlevel of expression in midbrain and thalamus. The ratio between the long and short form is similar in RT-PCR reaction. In human and rodent, the same two spliced forms are known to be coupled to G(i)-type heterotrimeric GTP binding protein, thereby opening an inwardly rectifying potassium channel, GIRK1. When the canine DRD2L and DRD2S were heterologously expressed in Xenopus oocytes, both forms activated GIRK1 potassium channels through couplingwith Gi protein. This activation was dose-dependent, demonstrating its ligand specificity. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:23:03