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Titolo:
Puromycin resistance (pac) gene as a selectable marker in vaccinia virus
Autore:
Sanchez-Puig, JM; Blasco, R;
Indirizzi:
INIA, Dept Mejora Genet & Biotecnol, E-28040 Madrid, Spain INIA Madrid Spain E-28040 ejora Genet & Biotecnol, E-28040 Madrid, Spain
Titolo Testata:
GENE
fascicolo: 1, volume: 257, anno: 2000,
pagine: 57 - 65
SICI:
0378-1119(20001017)257:1<57:PR(GAA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOST RANGE SELECTION; MAMMALIAN-CELLS; STREPTOMYCES-ALBONIGER; EXPRESSION VECTORS; DOMINANT SELECTION; FOREIGN GENES; RECOMBINANTS; VIRULENCE; PHENOTYPE;
Keywords:
eukaryotic cloning vector; marker gene; poxvirus; puromycin acetyltransferase gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Blasco, R INIA, Dept Mejora Genet & Biotecnol, Ctra La Coruna Km 7, E-28040 Madrid, Spain INIA Ctra La Coruna Km 7 Madrid Spain E-28040 040 Madrid, Spain
Citazione:
J.M. Sanchez-Puig e R. Blasco, "Puromycin resistance (pac) gene as a selectable marker in vaccinia virus", GENE, 257(1), 2000, pp. 57-65

Abstract

The antibiotic puromycin, an inhibitor of protein synthesis, was shown to inhibit vaccinia virus (VV) replication. We evaluated the use of puromycin-resistance (pac) gene as a selectable marker in VV. A recombinant vaccinia virus expressing pac (VV-pac) under the control of a viral early/late promoter was constructed and characterized. VV-pac grew in the presence of puromycin at concentrations that were inhibitory for the parental VV and toxic for the cells. Isolation of recombinant VV usually relies on plaque purification under selective conditions. Because virus plaquing was not feasible under inhibitory puromycin concentration, a protocol based on serial passage of virus was devised. The usefulness of this procedure in selecting pac expressing viruses was tested by isolating a recombinant VV. (C) 2000 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 18:52:56