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Titolo:
The zinc finger protein schnurri nets as a smad partner in mediating the transcriptional response to decapentaplegic
Autore:
Dai, H; Hogan, C; Gopalakrishnan, B; Torres-Vazquez, J; Nguyen, M; Park, SB; Raftery, LA; Warrior, R; Arora, K;
Indirizzi:
Univ Calif Irvine, Dept Dev & Cell Biol, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 v & Cell Biol, Irvine, CA 92697 USA Univ So Calif, Dept Biol Sci, Program Mol Biol, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 ol Biol, Los Angeles, CA 90089 USA Massachusetts Gen Hosp, Cutaneous Biol Res Ctr, Charlestown, MA 02129 USA Massachusetts Gen Hosp Charlestown MA USA 02129 Charlestown, MA 02129 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 227, anno: 2000,
pagine: 373 - 387
SICI:
0012-1606(20001115)227:2<373:TZFPSN>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BETA FAMILY; MAJOR HISTOCOMPATIBILITY COMPLEX; DROSOPHILA ENDODERM INDUCTION; DPP MORPHOGEN GRADIENT; DNA-BINDING; SIGNALING PATHWAY; I RECEPTORS; FUNCTIONAL INTERACTION; GERM LAYERS; GENE;
Keywords:
Drosophila; BMP; Ultrabithorax;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Arora, K Univ Calif Irvine, Dept Dev & Cell Biol, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 Biol, Irvine, CA 92697 USA
Citazione:
H. Dai et al., "The zinc finger protein schnurri nets as a smad partner in mediating the transcriptional response to decapentaplegic", DEVELOP BIO, 227(2), 2000, pp. 373-387

Abstract

In Drosophila, a BMP-related ligand Decapentaplegic (Dpp) is essential forcell fate specification during embryogenesis and in imaginal disc development. Dpp signaling culminates in the phosphorylation and nuclear translocation of Mothers against dpp (Mad), a receptor-specific Smad that can bind DNA and regulate the transcription of Dpp-responsive genes. Genetic analysis has implicated Schnurri (Shn), a zinc finger protein that shares homology with mammalian transcription factors, in the Dpp signal transduction pathway. However, a direct role for Shn in regulating the transcriptional responseto Dpp has not been demonstrated. In this study we show that Shn acts as aDNA -binding Mad cofactor in the nuclear response to Dpp. Shn can bind DNAin a sequence-specific manner and recognizes sites within a well-characterized Dpp-responsive promoter element, the B enhancer of the Ultrabithorax (Ubx) gene. The Shn-binding sites are relevant for in vivo expression, sincemutations in these sites affect the ability of the enhancer to respond to Dpp. Furthermore we find that Shn and Mad can interact directly through discrete domains. To examine the relative contribution of the two proteins in the regulation of endogenous Dpp target genes we developed a cell culture assay and show that Shn and Mad act synergistically to induce transcription. Our results suggest that cooperative interactions between these two transcription factors could play an important role in the regulation of Dpp target genes. This is the first evidence that Dpp/BMP signaling in flies requires the direct interaction of Mad with a partner transcription factor, (C) 2000 Academic Press.

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Documento generato il 25/11/20 alle ore 01:07:31