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Titolo:
Non-aqueous capillary electrophoresis of biological samples after at-line solid-phase extraction
Autore:
Veraart, JR; Reinders, MC; Lingeman, H; Brinkman, UAT;
Indirizzi:
Vrije Univ Amsterdam, Dept Analyt Chem & Appl Spect, NL-1081 HV Amsterdam,Netherlands Vrije Univ Amsterdam Amsterdam Netherlands NL-1081 HV terdam,Netherlands
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 7-8, volume: 52, anno: 2000,
pagine: 408 - 412
SICI:
0009-5893(200010)52:7-8<408:NCEOBS>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIQUID-CHROMATOGRAPHY; ANTIDEPRESSANT DRUGS; URINE;
Keywords:
capillary electrophoresis; non-aqueous buffer; solid-phase extraction; tricyclic antidepressants; serum and urine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Veraart, JR TNO, Packaging Res Dept, POB 360, NL-3700 AJ Zeist, Netherlands TNO POB 360 Zeist Netherlands NL-3700 AJ J Zeist, Netherlands
Citazione:
J.R. Veraart et al., "Non-aqueous capillary electrophoresis of biological samples after at-line solid-phase extraction", CHROMATOGR, 52(7-8), 2000, pp. 408-412

Abstract

Solid-phase extraction (SPE) wets coupled at-line to capillary electrophoresis (CE) for the determination of a series of basic lest compounds (i.e. tricyclic antidepressants). The analysis was performed using a non-aqueous CE buffer, which resulted in baseline separation of all test compounds. Thisis in marked contrast with CE using aqueous buffers where hardly any separation was obtained either with or without micelles. The SPE procedure was used to remove simultaneously most of the water from the sample, because no direct analysis of aqueous samples is possible when a non-aqueous CE bufferis used. With the present method the antidepressants can be determined in both urine and serum. Analyte detectability is increased up to to-fold due to trace enrichment during the extraction process the limits of detection (LODs; UV 214 nm) are 30 - 300 ng mL(-1) in urine and 300 - 1000 ng mL(-1) in serum. The RSD values (n = 5) of the within-day and between-day precisionare below 9% and 11%, respectively. Therefore, the present procedure can be used for drug monitoring.

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Documento generato il 29/03/20 alle ore 18:18:11