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Titolo:
Acute changes of myocardial creatine kinase gene expression under beta-adrenergic stimulation
Autore:
Hammerschmidt, S; Bell, M; Buchler, N; Wahn, H; Remkes, H; Lohse, MJ; Neubauer, S;
Indirizzi:
Univ Wurzburg, Dept Med Pharmacol, D-97070 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97070 rmacol, D-97070 Wurzburg, Germany Univ Wurzburg, Dept Pharmacol, D-97070 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97070 rmacol, D-97070 Wurzburg, Germany
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
fascicolo: 3, volume: 1502, anno: 2000,
pagine: 471 - 480
SICI:
0925-4439(20001115)1502:3<471:ACOMCK>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDIOPATHIC DILATED CARDIOMYOPATHY; INDUCED CARDIAC-HYPERTROPHY; TISSUE-SPECIFIC EXPRESSION; CONGESTIVE HEART-FAILURE; C-FOS EXPRESSION; ADULT-RAT HEART; MESSENGER-RNAS; PROTOONCOGENE EXPRESSION; DEVELOPMENTAL REGULATION; DIASTOLIC DYSFUNCTION;
Keywords:
creatine kinase; gene expression; beta-adrenergic stimulation; isoproterenol; isolated heart;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Hammerschmidt, S Univ Leipzig, Med Klin 1, Johannisallee 32, D-04103 Leipzig, Germany Univ Leipzig Johannisallee 32 Leipzig Germany D-04103 ny
Citazione:
S. Hammerschmidt et al., "Acute changes of myocardial creatine kinase gene expression under beta-adrenergic stimulation", BBA-MOL BAS, 1502(3), 2000, pp. 471-480

Abstract

Creatine kinase (CK) plays a crucial role in myocardial energy metabolism. Alterations in CK gene expression are found in hypertrophied and failing heart, but the mechanisms behind these changes are unclear. This study teststhe hypothesis that increased adrenergic stimulation, which is observed inheart failure, induces changes of myocardial CK-activity, -isoenzyme distribution and -gene expression that are characteristic of the failing and hypertrophied heart. Isolated rat hearts were perfused (constant pressure of 80 mmHg) with red cell suspensions. Following a 20-min warm-up period, perfusion for 3 h with 10(-8) M (iso 3 h) or without (control 3 h) isoproterenolwas started or experiments were immediately terminated (control 0 h). Leftventricular tissue was analyzed for total CK-activity, CK-isoenzyme distribution and, by use of quantitative RT-PCR, for B-CK, M-CK, mito-CK and GAPDH- (as internal standard) mRNA. After beta -adrenergic stimulation (iso 3 h) but not after control perfusion (control 3 h) a roughly threefold increase in B-CK mRNA levels and a decrease in M-CK mRNA levels by 18% was found. There were no significant differences among the three groups in total CK-activity and in distribution of CK-MM, CK-BB, CK-MB and mito-CK. Thus, beta -adrenergic stimulation induces a switch in CK gene expression from M-CK to B-CK, which is characteristic for the hypertrophied and failing heart. Thismay be interpreted as an adaptive mechanism making energy transduction viaCK more efficient at times of increased metabolic demand. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:23:32