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Titolo:
Characterization of complex chromosomal abnormalities in B-cell lymphoma by a combined spectral karyotyping (SKY) analysis and fluorescence in situ hybridization (FISH) using a 14q telomere probe
Autore:
Kakazu, N; Kito, K; Hitomi, T; Oita, J; Nishida, K; Masuda, K; Miki, T; Abe, T;
Indirizzi:
Kyoto Prefectural Univ Med, Dept Hyg, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Hikone City Hosp, Dept Internal Med, Hikone, Japan Hikone City Hosp Hikone Japan ty Hosp, Dept Internal Med, Hikone, Japan Kyoto Prefectural Univ Med, Dept Internal Med 3, Kyoto 602, Japan Kyoto Prefectural Univ Med Kyoto Japan 602 ernal Med 3, Kyoto 602, Japan Kyoto Prefectural Univ Med, Dept Urol, Kyoto 602, Japan Kyoto Prefectural Univ Med Kyoto Japan 602 , Dept Urol, Kyoto 602, Japan Tokyo Med & Dent Univ, Dept Internal Med 1, Tokyo 113, Japan Tokyo Med & Dent Univ Tokyo Japan 113 t Internal Med 1, Tokyo 113, Japan
Titolo Testata:
AMERICAN JOURNAL OF HEMATOLOGY
fascicolo: 4, volume: 65, anno: 2000,
pagine: 291 - 297
SICI:
0361-8609(200012)65:4<291:COCCAI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMA; TRANSLOCATION; REARRANGEMENTS; MALIGNANCIES;
Keywords:
spectral karyotyping (SKY); fluorescence in situ hybridization (FISH); complex chromosomal abnormalities; B-cell lymphoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Abe, T Kyoto Prefectural Univ Med, Dept Hyg, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 oto 6028566, Japan
Citazione:
N. Kakazu et al., "Characterization of complex chromosomal abnormalities in B-cell lymphoma by a combined spectral karyotyping (SKY) analysis and fluorescence in situ hybridization (FISH) using a 14q telomere probe", AM J HEMAT, 65(4), 2000, pp. 291-297

Abstract

We report a case of non-Hodgkin's lymphoma of unknown origin with invasioninto bone marrow and brain, This case showed complex chromosomal abnormalities, including five clonal marker chromosomes (mar) and four additional materials of unknown origin (add) that could not be identified by means of conventional G-banding. Spectral karyotyping (SKY) analysis could not only determine the origin and organization of all thus far unidentified structuralchromosomal abnormalities but also detect two cryptic unbalanced translocations, which had been erroneously considered to be normal on the basis of G-banding analysis, and correct one abnormality misidentified by G banding. Among these abnormalities, we identified the new partner site of the 14q32 translocation, 22q13, and the jumping translocations involving 2p23 as a new donor chromosome, Furthermore, by using fluorescence in situ hybridization (FISH) with the probes specific for the 14q telomere, we could identify the unbalanced translocation of t(3;14)(q27;q32), which had been erroneouslyconsidered to be normal chromosome 3 on the basis of not only G-banding but also of SKY analysis. This translocation is one of the mast frequent chromosomal abnormalities in B-cell lymphoma, especially diffuse large cell lymphoma, After SKY and FISH analysis, the original descriptions in the G-bandkaryotype were modified for a total of 13 chromosomes. The combination of SKY and FISH using the 14q telomere probe was therefore considered very useful for the characterization of complex cytogenetic cases in B-cell lymphoma, (C) 2000 Wiley-Liss, Inc.

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Documento generato il 04/07/20 alle ore 03:46:20