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Titolo:
HTLV type 1 tax transduction in microglial cells and astrocytes by lentiviral vectors
Autore:
Wrzesinski, S; Seguin, R; Liu, YX; Domville, S; Planelles, V; Massa, P; Barker, E; Antel, J; Feuer, G;
Indirizzi:
SUNY Upstate Med Univ, Dept Microbiol & Immunol, Syracuse, NY 13210 USA SUNY Upstate Med Univ Syracuse NY USA 13210 munol, Syracuse, NY 13210 USA McGill Univ, MNI, Neuroimmunol Unit, Montreal, PQ H3A 2B4, Canada McGill Univ Montreal PQ Canada H3A 2B4 Unit, Montreal, PQ H3A 2B4, Canada Univ Rochester, Ctr Canc, Dept Med & Microbiol & Immunol, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 & Immunol, Rochester, NY 14642 USA
Titolo Testata:
AIDS RESEARCH AND HUMAN RETROVIRUSES
fascicolo: 16, volume: 16, anno: 2000,
pagine: 1771 - 1776
SICI:
0889-2229(20001101)16:16<1771:HT1TTI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
I-ASSOCIATED MYELOPATHY; TROPICAL SPASTIC PARAPARESIS; VIRUS TYPE-I; CEREBROSPINAL-FLUID; GENE-TRANSFER; EXPRESSION; INDUCTION; IDENTIFICATION; RETROVIRUS; EFFICIENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Feuer, G SUNY Upstate Med Univ, Dept Microbiol & Immunol, 750 E Adams St, Syracuse,NY 13210 USA SUNY Upstate Med Univ 750 E Adams St Syracuse NY USA 13210 10 USA
Citazione:
S. Wrzesinski et al., "HTLV type 1 tax transduction in microglial cells and astrocytes by lentiviral vectors", AIDS RES H, 16(16), 2000, pp. 1771-1776

Abstract

Infection with human T cell leukemia virus type 1 (HTLV-1) can result in the development of HAM/TSP, a nonfatal, chronic inflammatory disease involving neuronal degeneration and demyelination of the central nervous system, Elevated levels of the proinflammatory cytokines tumor necrosis factor alpha(TNF-alpha), interleukin-6 (IL-6), and IL-1 observed in the cerebrospinal fluid of HAM-TSP patients suggest that cytokine dysregulation within the CNS is involved in neuropathogenesis. HTLV-1 infection and enhanced expression of TNF-alpha by microglial cells, astrocytes, and macrophages has been hypothesized to lead to the destruction of myelin and oligodendrocytes in theCNS, Although the association of HTLV-2 infection and development of neurological disease is more tenuous, HTLV-2 has also been found to be associated with peripheral neuropathies, To investigate the roles of HTLV Taxi and Tart in the induction of cytokine disregulation in these cell types, we are currently developing gene delivery vectors based on human immunodeficiency virus type-1 (HIV-1) capable of stably coexpressing the HTLV-1 or -2 tax and eGFP reporter genes in primary human cells. Transduction frequencies of up to 50%, as assessed by eGFP expression, can be achieved in human monocyte-derived macrophages and in explanted cultures of human microglia, Preliminary data suggest that Taxi expression is sufficient to up-regulate the proinflammatory cytokine profile in explanted human microglial cells. Future experiments will compare and evaluate the effect of tax(1) and tax(2) gene expression on the cellular proinflammatory cytokine expression profile, as well as demonstrate the effects of transducing human fetal astrocytes and PBMC-derived macrophages.

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Documento generato il 19/09/20 alle ore 20:56:09