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Titolo:
Signal through gp130 activated by soluble interleukin (IL)-6 receptor (R) and IL-6 or IL-6R/IL-6 fusion protein enhances ex vivo expansion of human peripheral blood-derived hematopoietic progenitors
Autore:
Kimura, T; Wang, JF; Minamiguchi, H; Fujiki, H; Harada, S; Okuda, K; Kaneko, H; Yokota, S; Yasukawa, K; Abe, T; Sonoda, Y;
Indirizzi:
Kyoto Prefectural Univ Med, Dept Hyg, Kamigyo Ku, Kyoto 602, Japan Kyoto Prefectural Univ Med Kyoto Japan 602 Kamigyo Ku, Kyoto 602, Japan Kyoto Prefectural Univ Med, Dept Surg Gastroenterol, Kamigyo Ku, Kyoto 602, Japan Kyoto Prefectural Univ Med Kyoto Japan 602 Kamigyo Ku, Kyoto 602, Japan Kyoto Prefectural Univ Med, Dept Internal Med 3, Kamigyo Ku, Kyoto 602, Japan Kyoto Prefectural Univ Med Kyoto Japan 602 Kamigyo Ku, Kyoto 602, Japan Shiga Univ Med Sci, Dept Internal Med 2, Shiga, Japan Shiga Univ Med Sci Shiga Japan d Sci, Dept Internal Med 2, Shiga, Japan Tosoh Corp, Tokyo Res Ctr, Kanagawa, Japan Tosoh Corp Kanagawa JapanTosoh Corp, Tokyo Res Ctr, Kanagawa, Japan
Titolo Testata:
STEM CELLS
fascicolo: 6, volume: 18, anno: 2000,
pagine: 444 - 452
SICI:
1066-5099(2000)18:6<444:STGABS>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM-CELL FACTOR; HIGH-DOSE CHEMOTHERAPY; LONG-TERM GENERATION; FLT3 LIGAND; IN-VITRO; C-KIT; NEGATIVE REGULATION; THROMBOPOIETIN; PROLIFERATION; MICE;
Keywords:
IL-6R/IL-6 fusion protein; soluble IL-6R; gp130; PB-derived CD34(+)IL-6R(-) cells; ex vivo expansion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Sonoda, Y Kyoto Prefectural Univ Med, Dept Hyg, Kamigyo Ku, Kyoto 602, Japan Kyoto Prefectural Univ Med Kyoto Japan 602 u, Kyoto 602, Japan
Citazione:
T. Kimura et al., "Signal through gp130 activated by soluble interleukin (IL)-6 receptor (R) and IL-6 or IL-6R/IL-6 fusion protein enhances ex vivo expansion of human peripheral blood-derived hematopoietic progenitors", STEM CELLS, 18(6), 2000, pp. 444-452

Abstract

This study was designed to investigate the effects of a combination of soluble interleukin (sIL)-6 receptor (R) and IL-6 on the ex vivo expansion of human peripheral blood (PB)-derived hematopoietic progenitor cells in a short-term serum-free liquid suspension culture system, using PB-derived CD34()IL-6R(+/-) cells as a target, In combination with stem cell factor (SCF),IL-3, and sIL-6R/IL-6, the expansion efficiency (EE) for granulocyte/macrophage colony-forming unit (CFU-GM) reached a peak level on day 10 of incubation, On the other hand, the EE for erythroid burst (BFU-E) and mixed colony-forming unit (CFU-Mix) reached a peak level on day 7 of incubation. Among the cytokine combinations tested, SCF + IL-3 + sIL-6R/IL-6 + flt3 ligand (FL) most effectively expanded CFU-GM and CFU-Mix, The maximum EEs for CFU-GMand CFU-Mix were 208-fold and 42-fold, respectively. While the EE for BFU-E was 70-90-fold in the presence of SCF + IL-3 + sIL-6R/IL-6, FL significantly augmented the EE for CFU-GM and CFU-Mix. In contrast, thrombopoietin (TPO) significantly augmented the EE for CFU-Mix, Interestingly, in combination with IL-3 and SCF, newly generated IL-6R/IL-6 fusion protein (FP) expanded PB-derived BFU-E and CFU-Mix twice more effectively than a combination of sIL-6R and IL-6, These results demonstrated that human PB-derived committed progenitors were effectively expanded in vitro using sIL-6R/IL-6 or FP, in combination with IL-3, SCF and/or FL or TPO, and that FP may transduce astronger intracellular signal than a combination of sIL-6R and IL-6.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:21:39