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Titolo:
PCR detection of human enteric viruses in bathing areas, waste waters and human stools in southwestern France
Autore:
Schvoerer, E; Bonnet, F; Dubois, V; Cazaux, G; Serceau, R; Fleury, HJA; Lafon, ME;
Indirizzi:
Univ Bordeaux 2, Virol Lab, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ol Lab, F-33076 Bordeaux, France Direct Dept Affaires Sanit & Sociales Gironde, F-33062 Bordeaux, France Direct Dept Affaires Sanit & Sociales Gironde Bordeaux France F-33062 ce Inst Europeen Environm Bordeaux, F-33000 Bordeaux, France Inst Europeen Environm Bordeaux Bordeaux France F-33000 Bordeaux, France
Titolo Testata:
RESEARCH IN MICROBIOLOGY
fascicolo: 8, volume: 151, anno: 2000,
pagine: 693 - 701
SICI:
0923-2508(200010)151:8<693:PDOHEV>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUND-STRUCTURED VIRUSES; HEPATITIS-A VIRUS; REVERSE-TRANSCRIPTASE PCR; POLYMERASE CHAIN-REACTION; VIRAL GASTROENTERITIS; SEMINESTED PCR; NORWALK-VIRUS; FOOD-BORNE; ENTEROVIRUSES; OUTBREAKS;
Keywords:
PCR analysis; enteric viruses; Norwalk-like viruses; environmental water; diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Schvoerer, E Univ Bordeaux 2, Virol Lab, 146 Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Leo Saignat Bordeaux France F-33076 ance
Citazione:
E. Schvoerer et al., "PCR detection of human enteric viruses in bathing areas, waste waters and human stools in southwestern France", RES MICROB, 151(8), 2000, pp. 693-701

Abstract

Detection of human pathogenic viruses by molecular techniques might be suitable for identifying viral pollution in environmental waters and for improving diagnosis in patients. Environmental samples were taken from bathing areas and sewage treatment plants in southwestern France. Small volume samples (50 muL) were tested. Five groups of enteric pathogenic viruses were studied: enteroviruses, Norwalk-like viruses (NLVs), hepatitis A virus, rotaviruses and adenoviruses. Moreover, human samples were tested for NLV. After extraction of viral nucleic acids (Boom's procedure), a nested polymerase chain reaction was conducted before hybridization. Five bathing waters out of 26 were positive for one viral group, without systematic association withbacterial contamination. Eight sewage plant samples out of 13 were positive for at least one viral group. Seven patients out of 45 were NLV-positive. Molecular techniques allow efficient screening of viral contamination in environmental waters and the study of NLV molecular epidemiology. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:20:33