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Titolo:
Bacterial community DNA extracted from soils polluted with heavy metals
Autore:
Kozdroj, J; van Elsas, JD;
Indirizzi:
Silesian Univ, Dept Microbiol, PL-40032 Katowice, Poland Silesian Univ Katowice Poland PL-40032 robiol, PL-40032 Katowice, Poland Res Inst Plant Protect, IPO, DLO, NL-6700 GW Wageningen, Netherlands Res Inst Plant Protect Wageningen Netherlands NL-6700 GW en, Netherlands
Titolo Testata:
POLISH JOURNAL OF ENVIRONMENTAL STUDIES
fascicolo: 5, volume: 9, anno: 2000,
pagine: 403 - 407
SICI:
1230-1485(2000)9:5<403:BCDEFS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MICROBIAL DNA; RIBOSOMAL-RNA; RAPID METHOD; HUMIC ACIDS; PCR; TRANSFORMATION; AMPLIFICATION; DIVERSITY; SEDIMENTS;
Keywords:
DNA extraction; PCR amplification; heavy metals; polluted soil;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kozdroj, J Silesian Univ, Dept Microbiol, Jagiellonska 28, PL-40032 Katowice, Poland Silesian Univ Jagiellonska 28 Katowice Poland PL-40032 Poland
Citazione:
J. Kozdroj e J.D. van Elsas, "Bacterial community DNA extracted from soils polluted with heavy metals", POL J ENV S, 9(5), 2000, pp. 403-407

Abstract

This paper describes the application of two direct and one indirect methods for the extraction of microbial community DNA from soils polluted with heavy metals. DNA was extracted directly from soil using a gentle method based on soil incubation at 37 degreesC with proteinase K and SDS, or the method was modified by the addition of a bead beating step. The indirect approach was based on the RNA/DNA extraction method. The level of soil contamination did not affect the yields of DNA extracted and PCR amplification of the target DNA. The results indicate that the DNA obtained by the applied protocols was sufficiently pure for further molecular analyses.

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Documento generato il 01/10/20 alle ore 07:05:45