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Titolo:
SCLERODERMA LUNG FIBROBLASTS EXHIBIT ELEVATED AND DYSREGULATED TYPE-ICOLLAGEN BIOSYNTHESIS
Autore:
XU SW; DENTON CP; MCWHIRTER A; BOUGHARIOS G; ABRAHAM DJ; DUBOIS RM; BLACK CM;
Indirizzi:
ROYAL FREE HOSP,ACAD UNIT RHEUMATOL,POND ST LONDON NW3 2QG ENGLAND ROYAL FREE HOSP,ACAD UNIT RHEUMATOL LONDON NW3 2QG ENGLAND UNIV LONDON KINGS COLL LONDON WC2R 2LS ENGLAND UPPSALA UNIV UPPSALA SWEDEN ROYAL POSTGRAD MED SCH LONDON ENGLAND NATL INST MED RES,MRC LONDON NW7 1AA ENGLAND ROYAL BROMPTON HOSP LONDON SW3 6LY ENGLAND
Titolo Testata:
Arthritis and rheumatism
fascicolo: 7, volume: 40, anno: 1997,
pagine: 1237 - 1244
SICI:
0004-3591(1997)40:7<1237:SLFEEA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRONCHOALVEOLAR LAVAGE FLUID; SYSTEMIC-SCLEROSIS; LATTICES; GROWTH; PROLIFERATION; EXPRESSION; FEATURES; FIBROSIS; THROMBIN; DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
S.W. Xu et al., "SCLERODERMA LUNG FIBROBLASTS EXHIBIT ELEVATED AND DYSREGULATED TYPE-ICOLLAGEN BIOSYNTHESIS", Arthritis and rheumatism, 40(7), 1997, pp. 1237-1244

Abstract

Objective. To examine whether scleroderma lung fibroblasts show a pattern of aberrant type I collagen (CI) biosynthesis similar to that observed previously in studies of dermal fibroblasts in this disease. Methods. CI secretion and steady-state pro alpha 1(I) collagen messenger RNA (mRNA) levels and COL1A2 gene activation were examined in fibroblasts grown from lung biopsy specimens obtained from 16 scleroderma patients with lung fibrosis and from 10 histologically normal lung specimens (controls). The effect of culture in a 3-dimensional (3-D) CI gel matrix culture on CI mRNA levels was also examined. Results. The mean (/-SEM) collagen secretion in monolayer culture for scleroderma lung fibroblasts was 90.9 +/- 56 ng/ml/10(6) cells, significantly greater (P< 0.05) than controls (40.2 +/- 17.5). Pro alpha 1(I) collagen mRNA levels in monolayer cultures were higher in scleroderma (mean +/- SEM collagen:GAPDH ratio 3.7 +/- 0.9) compared with control (1.9 +/- 0.8) lung fibroblasts. Transient expression assays confirmed that genes coding for CI are transcriptionally activated in scleroderma lung fibroblasts compared with control strains. Although all lung fibroblasts induced equivalent contraction of 3-D CI gel matrices, scleroderma strains failed to show a reduction in steady-state pro alpha 1(I) collagen mRNA levels in gel culture. Conclusion. We have demonstrated elevated CI biosynthesis and impaired mRNA down-regulation for CI by scleroderma lung fibroblasts. These properties are likely to be highly relevant to the pathogenesis of scleroderma-associated lung fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 05:43:48