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Titolo:
Streptogramin-based gene regulation systems for mammalian cells
Autore:
Fussenegger, M; Morris, RP; Fux, C; Rimann, M; von Stockar, B; Thompson, CJ; Bailey, JE;
Indirizzi:
ETH Zurich, Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 chnol, CH-8093 Zurich, Switzerland Univ Basel, Bioctr, Dept Microbiol, CH-4056 Basel, Switzerland Univ BaselBasel Switzerland CH-4056 crobiol, CH-4056 Basel, Switzerland
Titolo Testata:
NATURE BIOTECHNOLOGY
fascicolo: 11, volume: 18, anno: 2000,
pagine: 1203 - 1208
SICI:
1087-0156(200011)18:11<1203:SGRSFM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIDRUG-RESISTANCE GENE; HAMSTER OVARY CELLS; STREPTOMYCES-PRISTINAESPIRALIS; ERYTHROPOIETIN DELIVERY; ENCAPSULATED MYOBLASTS; EXPRESSION; MICE; QUINUPRISTIN/DALFOPRISTIN; PRODUCTIVITY; RECEPTOR;
Keywords:
gene therapy; tissue engineering; Streptomyces; Synercid; erythropoietin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Fussenegger, M ETH Zurich, Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 Zurich, Switzerland
Citazione:
M. Fussenegger et al., "Streptogramin-based gene regulation systems for mammalian cells", NAT BIOTECH, 18(11), 2000, pp. 1203-1208

Abstract

Here we describe repressible (PipOFF) as well as inducible (PipON) systemsfor regulated gene expression in mammalian cells, based on the repressor Pip (pristinamycin-induced protein), which is encoded by the streptogramin resistance operon of Streptomyces coelicolor. Expression of genes placed under control of these systems was responsive to clinically approved antibiotics belonging to the streptogramin group (pristinamycin, virginiamycin, and Synercid). The versatility of these systems was demonstrated by streptogramin-regulated expression of mouse erythropoietin (EPO), human placental secreted alkaline phosphatase (SEAP), or green fluorescent protein (GFP) in diverse cell lines (BHK, CHO, HeLa, and mouse myoblasts). Analysis of isogenicconstructs in CHO cells demonstrated the PipOFF system gave lower background and higher induction ratios than the widely used tetracycline-repressible (TetOFF) expression systems. The streptogramin-based expression technology was functionally compatible with the TetOFF system, thus enabling the selective use of different antibiotics to independently control two different gene activities in the same cell.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:58:19