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Titolo:
Functional genomic analysis of cell division in C-elegans using RNAi of genes on chromosome III
Autore:
Gonczy, P; Echeverri, C; Oegema, K; Coulson, A; Jones, SJM; Copley, RR; Duperon, J; Oegema, J; Brehm, M; Cassin, E; Hannak, E; Kirkham, M; Pichler, S; Flohrs, K; Goessen, A; Leidel, S; Alleaume, AM; Martin, C; Ozlu, N; Bork, P; Hyman, AA;
Indirizzi:
European Mol Biol Lab, D-69117 Heidelberg, Germany European Mol Biol Lab Heidelberg Germany D-69117 117 Heidelberg, Germany Sanger Ctr, Cambridge CB10 1SA, England Sanger Ctr Cambridge England CB10 1SA r Ctr, Cambridge CB10 1SA, England British Columbia Canc Res Ctr, Genome Sequence Ctr, Vancouver, BC V5Z 4E6,Canada British Columbia Canc Res Ctr Vancouver BC Canada V5Z 4E6 V5Z 4E6,Canada Max Planck Inst Cell Biol & Genet, D-01307 Dresden, Germany Max Planck Inst Cell Biol & Genet Dresden Germany D-01307 esden, Germany
Titolo Testata:
NATURE
fascicolo: 6810, volume: 408, anno: 2000,
pagine: 331 - 336
SICI:
0028-0836(20001116)408:6810<331:FGAOCD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAENORHABDITIS-ELEGANS; MICROTUBULE ORGANIZATION; CYTOPLASMIC DYNEIN; SPINDLE FORMATION; GERM-LINE; PROTEIN; EMBRYOS; POLARITY; RAN; CYTOKINESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Gonczy, P Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland Swiss Inst Expt Canc Res Epalinges Switzerland CH-1066 zerland
Citazione:
P. Gonczy et al., "Functional genomic analysis of cell division in C-elegans using RNAi of genes on chromosome III", NATURE, 408(6810), 2000, pp. 331-336

Abstract

Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of similar to 96% ofthe similar to2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (similarto6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome IIIthat are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that similar to 47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:00:55