Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Stability indicating HPTLC determination of timolol maleate as bulk drug and in pharmaceutical preparations
Autore:
Kulkarni, SP; Amin, PD;
Indirizzi:
Univ Bombay, Dept Chem Technol Autonomous, Pharmaceut Sci & Technol Div, Bombay 400019, Maharashtra, India Univ Bombay Bombay Maharashtra India 400019 ay 400019, Maharashtra, India
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 6, volume: 23, anno: 2000,
pagine: 983 - 987
SICI:
0731-7085(200011)23:6<983:SIHDOT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ULTRAVIOLET DETECTION; HUMAN-PLASMA; URINE;
Keywords:
timolol maleate; HPTLC; degradation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Amin, PD Univ Bombay, Dept Chem Technol Autonomous, Pharmaceut Sci & Technol Div, Nathalai Parikh Marg, Bombay 400019, Maharashtra, India Univ BombayNathalai Parikh Marg Bombay Maharashtra India 400019
Citazione:
S.P. Kulkarni e P.D. Amin, "Stability indicating HPTLC determination of timolol maleate as bulk drug and in pharmaceutical preparations", J PHARM B, 23(6), 2000, pp. 983-987

Abstract

Timolol was the first beta blocker to be used as an anti-glaucoma agent and to date remains as the standard because none of the newer beta blockers were found to be more effective. The high performance thin layer chromatographic method of analysis of timolol maleate is reported. The mobile phase selected was ethyl acetate-methanol-isopropyl alcohol-ammonia (25%) (80:20:2:1, v/v/v/v). The calibration curve of the drug was linear in the range of 100-600 ng. The spectrodensitometric analysis was carried out at 294 nm. Themean (+/- RSD) values of slope, correlation coefficient and intercept were2487.3 ( +/- 0.9), 0.996 ( +/- 0.081) and 90463 (+/- 1.1), respectively. The system precision and the method precision were excellent with an RSD of 2.8 and 1.004, respectively. The limits of detection and quantitation were 10 and 40 ng, respectively. The mean percent recovery was found to be 98.6. Timolol maleate was degraded by exposing the drug to heat, acid and base. The degraded products were found to be well separated from the pure drug with significantly different R-f values suggesting a stability indicating analysis method for quantification of timolol maleate in pharmaceutical preparations and as bulk drug. The method was utilized to analyze timolol maleatefrom conventional eye drops and novel sustained release solid polymeric ocular inserts and oral preparations. The reported method is simple, selective, precise, accurate, time saving and economic as compared to reported HPLCmethods. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:43:51