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Titolo:
Developmental expression of muscarinic acetylcholine receptors in chick retina: Selective induction of M-2 muscarinic receptor expression in ovo by afactor secreted by Muller glial cells
Autore:
Belmonte, KE; McKinnon, LA; Nathanson, NM;
Indirizzi:
Univ Washington, Sch Med, Dept Pharmacol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Pharmacol, Seattle, WA 98195 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 22, volume: 20, anno: 2000,
pagine: 8417 - 8425
SICI:
0270-6474(20001115)20:22<8417:DEOMAR>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMACRINE CELLS; RABBIT RETINA; VISUAL-SYSTEM; RELEASE; IDENTIFICATION; LOCALIZATION; INHIBITION; WAVES; LINE; GENE;
Keywords:
muscarinic; Muller glia; reporter gene; retina; chick; development; neurotrophic factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Nathanson, NM Univ Washington, Sch Med, Dept Pharmacol, Box 357750, Seattle, WA 98195 USA Univ Washington Box 357750 Seattle WA USA 98195 WA 98195 USA
Citazione:
K.E. Belmonte et al., "Developmental expression of muscarinic acetylcholine receptors in chick retina: Selective induction of M-2 muscarinic receptor expression in ovo by afactor secreted by Muller glial cells", J NEUROSC, 20(22), 2000, pp. 8417-8425

Abstract

Muscarinic acetylcholine receptors (mAChRs) play an important role in signal processing in the retina. We have used subtype-specific antibodies to identify the changes in the localization of mAChR expression during embryonicdevelopment of the retina in vivo and their relationship to the changes inmAChRs in retinal cells in culture. We have demonstrated previously that treatment of fresh retinal cultures with conditioned media from mature retinal cultures specifically induces expression of the M-2 mAChR (McKinnon et al., 1998). We show that the M-2-inducing activity, which we tentatively have called MARIA (muscarinic acetylcholine receptor-inducing activity) is produced by Muller glial cells in culture, because significant activity can befound in media conditioned by essentially neuron-free cultures of Muller glia, as well as by a Muller glial cell line but not several neuroblastoma cell lines. We also demonstrate that the appearance of the M2 receptor in vivo occurs concomitantly with the appearance of significant numbers of Muller glial cells in the developing retina. Furthermore, the administration of crude or partially purified preparations of MARIA to developing chick embryos in ovo induces precocious expression of M-2 mAChRs in the appropriate cell types in the retina. These results show that a factor secreted by cultured retinal Muller glia can regulate M-2 mAChR expression in vivo and in vitro and suggest that the secretion of MARIA by Muller glia in vivo may be responsible for the normal induction of M-2 mAChR expression during embryonicdevelopment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 21:31:49