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Titolo:
Intracellular localization and isoform expression of the voltage-dependentanion channel (VDAC) in normal and dystrophic skeletal muscle
Autore:
Massa, R; Marlier, LN; Martorana, A; Cicconi, S; Pierucci, D; Giacomini, P; De Pinto, V; Castellani, L;
Indirizzi:
Univ Roma Tor Vergata, Dipartimento Neurosci, Rome, Italy Univ Roma Tor Vergata Rome Italy ta, Dipartimento Neurosci, Rome, Italy Univ Roma Tor Vergata, Dipartimento Med Interna, Mol Med Lab, Rome, Italy Univ Roma Tor Vergata Rome Italy Med Interna, Mol Med Lab, Rome, Italy IRCCS Osped S Lucia, Rome, Italy IRCCS Osped S Lucia Rome ItalyIRCCS Osped S Lucia, Rome, Italy CNR, Ist Med Sperimentale, I-00137 Rome, Italy CNR Rome Italy I-00137CNR, Ist Med Sperimentale, I-00137 Rome, Italy Univ Rome La Sapienza, Neurol Clin 2, Rome, Italy Univ Rome La Sapienza Rome Italy a Sapienza, Neurol Clin 2, Rome, Italy Univ Catania, Dipartimento Sci Chim, Lab Biochim & Biol Mol, I-95125 Catania, Italy Univ Catania Catania Italy I-95125 im & Biol Mol, I-95125 Catania, Italy INFM, Sez B, Rome, Italy INFM Rome ItalyINFM, Sez B, Rome, Italy
Titolo Testata:
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
fascicolo: 5, volume: 21, anno: 2000,
pagine: 433 - 442
SICI:
0142-4319(200007)21:5<433:ILAIEO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER MITOCHONDRIAL-MEMBRANE; SARCOPLASMIC-RETICULUM; MDX-MOUSE; OXIDATIVE-PHOSPHORYLATION; SUBCELLULAR-LOCALIZATION; MUSCULAR-DYSTROPHY; CREATINE-KINASE; HUMAN PORIN; IN-VITRO; PERMEABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Massa, R Univ Roma Tor Vergata, Dipartimento Neurosci, Rome, Italy Univ Roma Tor Vergata Rome Italy timento Neurosci, Rome, Italy
Citazione:
R. Massa et al., "Intracellular localization and isoform expression of the voltage-dependentanion channel (VDAC) in normal and dystrophic skeletal muscle", J MUSCLE R, 21(5), 2000, pp. 433-442

Abstract

Voltage-dependent anion channels (VDACs) are a family of pore-forming proteins encoded by different genes, with at least three protein products expressed in mammalian tissues. The major recognized functional role of VDACs isto permit the almost free permeability of the outer mitochondrial membrane(OMM). Although VDAC1 is the best known among VDAC isoforms, its exclusively mitochondrial location is still debated. Therefore, we have measured itsco-localization with markers of cellular organelles or compartments in skeletal muscle fibers by single or double immunofluorescence and traditional as well as confocal microscopy. Our results show that VDAC1 immunoreactivity corresponds to mitochondria and sarcoplasmic reticulum, while sarcolemmalreactivity, previously reported, was not observed. Since VDAC1 has been suggested to be involved in the control of oxidative phosphorylation, we sought for possible gene regulation of VDAC1, VDAC2 and VDAC3 in skeletal muscle of the dystrophin-deficient mdx mouse, which suffers of an impaired control of energy metabolism. Our results show that, while VDAC1 mRNA and protein and VDAC2 mRNA are normally expressed, VDAC3 mRNA is markedly down-regulated in mdx mouse muscle at different ages (before, during and after the outburst of myofiber necrosis). This finding suggests a possible involvement of VDAC3 expression in the early pathogenic events of the mdx muscular dystrophy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:56:09