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Titolo:
MODULATION OF P53, WAF1 P21 AND BCL-2 EXPRESSION DURING RETINOIC ACID-INDUCED DIFFERENTIATION OF NB4 PROMYELOCYTIC CELLS/
Autore:
BOCCHIA M; XU Q; WESLEY U; XU Y; KORONTSVIT T; LOGANZO F; ALBINO AP; SCHEINBERG DA;
Indirizzi:
MEM SLOAN KETTERING CANC CTR,LEUKEMIA SERV,1275 YORK AVE NEW YORK NY 10021 MEM SLOAN KETTERING CANC CTR,LEUKEMIA SERV NEW YORK NY 10021
Titolo Testata:
Leukemia research
fascicolo: 5, volume: 21, anno: 1997,
pagine: 439 - 447
SICI:
0145-2126(1997)21:5<439:MOPWPA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIN-DEPENDENT KINASES; LEUKEMIA-CELLS; INHIBITS APOPTOSIS; PROTEIN; INDUCTION; DEATH; P21; WAF1/CIP1; GENE; MUTATIONS;
Keywords:
NB4 CELLS; RETINOIC ACID; P53; WAF1/P21; BCL-2; APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Bocchia et al., "MODULATION OF P53, WAF1 P21 AND BCL-2 EXPRESSION DURING RETINOIC ACID-INDUCED DIFFERENTIATION OF NB4 PROMYELOCYTIC CELLS/", Leukemia research, 21(5), 1997, pp. 439-447

Abstract

The NB4 cell line, established from a patient with APL, carries the t(15;17) and undergoes differentiation along the granulocytic pathway when exposed to retinoic acid (RA). The NB4 cell line was used as a model for exploring the expression of genes and proteins implicated in growth regulation, differentiation and apoptosis during treatment with RA. NB4 cells undergo a series of cytological and molecular alterationsduring RA treatment--Day 1: cell differentiation marked by an increase in CD11b is evident. Day 2: WAF1/p21 mRNA and then protein rise, though they drop 2 days later. Day 3: the percentage of cells in S phase begins to decrease and G1 arrest begins. Day 4: p53 mRNA level and then protein levels fall. Day 5: CD11b/BCL-2 double staining cells are markedly reduced. No signs of apoptosis were observed after up to 8 daysof treatment with RA. These results demonstrate that NB4 cells treated with RA rapidly differentiate and arrest at G1 phase concurrent withp53-independent WAF1/p21 induction; in addition, phenotypic differentiation appears to commence before changes in cell cycle progression. An explanation for the decrease in p53 as well as the lack of apoptosisimmediately after BCL-2 downregulation will require further study. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:41:42