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Titolo:
Determination of amino acid sequence and site of mRNA expression of four vitellins in the giant freshwater prawn, Macrobrachium rosenbergii
Autore:
Yang, WJ; Ohira, T; Tsutsui, N; Subramoniam, T; Huong, DTT; Aida, K; Wilder, MN;
Indirizzi:
Minist Agr Forestry & Fisheries, Japan Int Res Ctr Agr Sci, Tsukuba, Ibaraki 3058686, Japan Minist Agr Forestry & Fisheries Tsukuba Ibaraki Japan 3058686 8686, Japan Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 , Dept Aquat Biosci, Tokyo 1138657, Japan Univ Madras, Sch Life Sci, Dept Zool, Madras 600025, Tamil Nadu, India Univ Madras Madras Tamil Nadu India 600025 dras 600025, Tamil Nadu, India
Titolo Testata:
JOURNAL OF EXPERIMENTAL ZOOLOGY
fascicolo: 6, volume: 287, anno: 2000,
pagine: 413 - 422
SICI:
0022-104X(20001101)287:6<413:DOAASA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURE FEMALE HEMOLYMPH; WATER PRAWN; VITELLOGENIN SYNTHESIS; PROTEIN VITELLOGENIN; TERRESTRIAL ISOPOD; PENAEUS-MONODON; ARMADILLIDIUM-VULGARE; OVARIAN VITELLIN; YOLK PROTEINS; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Wilder, MN Minist Agr Forestry & Fisheries, Japan Int Res Ctr Agr Sci, 1-2Ohwashi, Tsukuba, Ibaraki 3058686, Japan Minist Agr Forestry & Fisheries 1-2 Ohwashi Tsukuba Ibaraki Japan 3058686
Citazione:
W.J. Yang et al., "Determination of amino acid sequence and site of mRNA expression of four vitellins in the giant freshwater prawn, Macrobrachium rosenbergii", J EXP ZOOL, 287(6), 2000, pp. 413-422

Abstract

Four major yolk proteins, designated as vitellins (Vns) Macr-VnA, B, C, and D, were extracted from mature ovaries of Macrobrachium rosenbergii. Thesewere purified to homogeneity by reversed-phase high performance liquid chromatography (HPLC) employing a unique separation system based on the hydrophobic properties of the Vn molecule. Using standard techniques of protein sequencing, more than 33 N-terminal and 57 internal amino acid residues weredetermined for each of the four Vns. The cDNA fragments encoding the four Vns were amplified by PCR using degenerate oligonucleotide primers derived from the N-terminal and internal amino acid sequences. These cDNA fragmentswere cloned, sequenced, and used as probes to examine the transcription ofmRNAs encoding the four Vns. Significant accumulations of these mRNAs wereobserved in female hepatopancreas only, while mRNA expression was not detected in male hepatopancreas or any other female tissue including ovary, subepidermal adipose tissue, gill, and muscle. This is the first occasion in Crustacea in which multiple Vns were demonstrated to be synthesized simultaneously in a single tissue. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 23:57:14