Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Architecture of Ca2+ channel pore-lining segments revealed by covalent modification of substituted cysteines
Autore:
Koch, SE; Bodi, I; Schwartz, A; Varadi, G;
Indirizzi:
Univ Cincinnati, Coll Med, Inst Mol Pharmacol & Biophys, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 Biophys, Cincinnati, OH 45267 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 44, volume: 275, anno: 2000,
pagine: 34493 - 34500
SICI:
0021-9258(20001103)275:44<34493:AOCCPS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-SPANNING SEGMENT; BINDING-SITE CREVICE; TRANSMEMBRANE CONDUCTANCE REGULATOR; DEPENDENT CALCIUM-CHANNEL; DOPAMINE D2 RECEPTOR; RAT PHEOCHROMOCYTOMA CELLS; NORMAL HUMAN HEART; ION PERMEATION; SODIUM-CHANNEL; BETA-SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Varadi, G Univ Cincinnati, Coll Med, Inst Mol Pharmacol & Biophys, 231 Bethesda Ave,Cincinnati, OH 45267 USA Univ Cincinnati 231 Bethesda Ave Cincinnati OH USA 45267 267 USA
Citazione:
S.E. Koch et al., "Architecture of Ca2+ channel pore-lining segments revealed by covalent modification of substituted cysteines", J BIOL CHEM, 275(44), 2000, pp. 34493-34500

Abstract

The cysteine accessibility method was used to explore calcium channel poretopology. Cysteine mutations were introduced into the SS1-SS2 segments of Motifs I-TV of the human cardiac L-type calcium channel, expressed in Xenopus oocytes and the current block by methanethiosulfonate compounds was measured. Our studies revealed that several consecutive mutants of motifs ZI and III are accessible to methanethiosulfonates, suggesting that these segments exist as random coils. Motif I cysteine mutants exhibited an intermittent sensitivity to these compounds, providing evidence for a beta -sheet secondary structure. Motif IV showed a periodic sensitivity, suggesting the presence of an cr-helix. These studies reveal that the SS1-SS2 segment repeat in each motif have non-uniform secondary structures. Thus, the channel architecture evolves as a highly distorted 4-fold pore symmetry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 09:27:09