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Titolo:
Effect of the gonadotropin-releasing hormone antagonist ganirelix on cyclic adenosine monophosphate accumulation of human granulosa-lutein cells
Autore:
Demirel, LC; Weiss, JM; Polack, S; Unlu, C; Diedrich, K; Ortmann, O;
Indirizzi:
Med Univ Lubeck, Dept Obstet & Gynecol, D-23538 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23538 Gynecol, D-23538 Lubeck, Germany Med Univ Ankara, Dept Obstet & Gynecol, Ankara, Turkey Med Univ Ankara Ankara Turkey ra, Dept Obstet & Gynecol, Ankara, Turkey
Titolo Testata:
FERTILITY AND STERILITY
fascicolo: 5, volume: 74, anno: 2000,
pagine: 1001 - 1007
SICI:
0015-0282(200011)74:5<1001:EOTGHA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RIBONUCLEIC-ACID; IN-VITRO FERTILIZATION; OVARIAN FOLLICLE; PROGESTERONE SECRETION; HYPOPHYSECTOMIZED RATS; INHIBITORY ACTIONS; GNRH RECEPTOR; AGONIST; STEROIDOGENESIS; EXPRESSION;
Keywords:
ganirelix; cAMP; hCG; granulosa cells; cumulus cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Ortmann, O Med Univ Lubeck, Dept Obstet & Gynecol, Ratzeburger Allee 160, D-23538 Lubeck, Germany Med Univ Lubeck Ratzeburger Allee 160 Lubeck Germany D-23538 y
Citazione:
L.C. Demirel et al., "Effect of the gonadotropin-releasing hormone antagonist ganirelix on cyclic adenosine monophosphate accumulation of human granulosa-lutein cells", FERT STERIL, 74(5), 2000, pp. 1001-1007

Abstract

Objective: To evaluate whether the GnRH antagonist ganirelix exerts an effect on cyclic adenosine monophosphate (cAMP) production of human granulosa-lutein (GL) cells in vitro. Design: In vitro cell culture study. Setting: Research laboratory of a university hospital. Patient(s): Mural GL and cumulus cells were obtained from 15 patients on whom controlled ovarian hyperstimulation was being performed for intracytoplasmic sperm injection treatment. Intervention(s): Mural CL and cumulus cells were cultured for 48 hours with and without 1 nM ganirelix or triptorelin. For the last 6 hours, the cells were either exposed to 1-5 IU hCG or left unstimulated. Main Outcome Measure(s): At the end of the culturing period, the intracellular and extracellular cAMP accumulations were measured by an I-125-scintillation proximity assay. Result(s): hCG induced dose-dependent increases in total cAMP accumulation. Stimulation with 1 IU/mL hCG resulted in 9-fold and 13-fold increases, and 5 IU/mL hCG resulted in 19-fold and 14-fold increases in total cAMP release from cumulus and mural GL cells, respectively. On the other hand, treatments with 1 nM GnRH antagonist ganirelix and 1 nM GnRH agonist triptorelin did not exert any significant changes on the basal and hCG-stimulated cAMP accumulation of mural GL cells and cumulus cells as compared with controls. Conclusion(s): Ganirelix does not influence basal and hCG-stimulated cAMP accumulation of human GL cells in vitro, cAMP is apparently not involved inthe mechanism of action of GnRH analogs in human ovary. (C) 2000 by American Society for Reproductive Medicine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 02:24:26