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Titolo:
A novel assay to demonstrate an intersection of the exocytic and endocyticpathways at early endosomes
Autore:
Laird, V; Spiess, M;
Indirizzi:
Univ Basel, Biozentrum, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 zentrum, CH-4056 Basel, Switzerland
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 260, anno: 2000,
pagine: 340 - 345
SICI:
0014-4827(20001101)260:2<340:ANATDA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MANNOSE 6-PHOSPHATE RECEPTORS; POLARIZED EPITHELIAL-CELLS; ASIALOGLYCOPROTEIN RECEPTOR; MDCK CELLS; CLATHRIN ADAPTERS; LYSOSOMAL-ENZYMES; PROTEIN COMPLEX; SORTING SIGNALS; AP-3 ADAPTER; FC-RECEPTORS;
Keywords:
asialoglycoprotein receptor; endocytosis; exocytosis; transferrin; basolateral sorting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Spiess, M Univ Basel, Biozentrum, Klingelbergstr 70, CH-4056 Basel, Switzerland Univ Basel Klingelbergstr 70 Basel Switzerland CH-4056 tzerland
Citazione:
V. Laird e M. Spiess, "A novel assay to demonstrate an intersection of the exocytic and endocyticpathways at early endosomes", EXP CELL RE, 260(2), 2000, pp. 340-345

Abstract

The mechanism of transport of membrane proteins from the trans-Golgi to the cell surface is still poorly understood. Previous studies suggested that basolateral membrane proteins, such as the transferrin receptor and the asialoglycoprotein receptor H1, take an indirect route to the plasma membrane via an intracellular, most likely endosomal intermediate. To define this compartment we developed a biochemical assay based on the very definition of endosomes. The assay is based on internalizing anti-H1 antibodies via the endocytic cycle of the receptor itself. Internalized antibody formed immune complexes with newly synthesized H1, which had been pulse-labeled with [S-35]sulfate and chased out of the trans-Golgi for a period of time that was insufficient for H1 to reach the surface. Hence, antibody capture occurred intracellularly. double-immunofluorescence labeling demonstrated that antibody-containing compartments also contained transferrin and thus correspondedto early and recycling endosomes. The results therefore demonstrate an intracellular intersection of the exocytic and endocytic pathways with implications for basolateral sorting. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 14:16:54