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Titolo:
Induction of the P450 reporter gene system bioassay by polycyclic aromatichydrocarbons in Ulsan Bay (South Korea) sediments
Autore:
Koh, CH; Kim, GB; Maruya, KA; Anderson, JW; Jones, JM; Kang, SG;
Indirizzi:
Skidaway Inst Oceanog, Savannah, GA 31411 USA Skidaway Inst Oceanog Savannah GA USA 31411 eanog, Savannah, GA 31411 USA Seoul Natl Univ, Dept Oceanog, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 anog, Seoul 151742, South Korea Columbia Analyt Sci, Vista, CA 92083 USA Columbia Analyt Sci Vista CA USA92083 ia Analyt Sci, Vista, CA 92083 USA
Titolo Testata:
ENVIRONMENTAL POLLUTION
fascicolo: 3, volume: 111, anno: 2001,
pagine: 437 - 445
SICI:
0269-7491(2001)111:3<437:IOTPRG>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHOXYRESORUFIN-O-DEETHYLASE; HEPATOMA-CELLS PLHC-1; POLYCHLORINATED-BIPHENYLS; ESTUARINE SEDIMENTS; TOXIC POTENCIES; EROD ACTIVITY; BIOMARKER; EXTRACTS; INHIBITION; EXPOSURE;
Keywords:
polycyclic aromatic hydrocarbons; bioassay; P450 RGS; sediment quality;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, GB Skidaway Inst Oceanog, 10 Ocean Sci Circle, Savannah, GA 31411 USASkidaway Inst Oceanog 10 Ocean Sci Circle Savannah GA USA 31411 SA
Citazione:
C.H. Koh et al., "Induction of the P450 reporter gene system bioassay by polycyclic aromatichydrocarbons in Ulsan Bay (South Korea) sediments", ENVIR POLLU, 111(3), 2001, pp. 437-445

Abstract

Polycyclic aromatic hydrocarbons (PAHs) and induction of the P450 reportergene system (RGS) for 6- and 16-h exposure periods were determined in organic extracts of Ulsan Bay (South Korea) sediments to assess the utility of this bioassay as a screening tool for PAH contamination. The sum of the concentrations of 23 individual PAHs in 30 sediment samples (Sigma PAH) based on GC-MS analysis ranged from 0.05 to 6.1 mug/g dry wt. P450 RGS fold induction ranged from 4.0 to 320 mug/g based on benzo[a]pyrene toxic equivalents(BaPEq). P450 RGS BaPEq and the 'chemical BaPEq', defined as the sum of the products of individual PAH concentrations and pre-determined toxic equivalency factors, exhibited very strong positive correlations with Sigma PAH (r(2)>0.90; P<0.001). Fold induction did not increase (and in some cases decreased) after the optimal incubation period (6 h) for PAHs, indicating thatother compounds known to induce the P450 RGS (e.g. chlorinated organics) were not present at levels effecting significant induction. This was supported by GC-ECD analysis where non-ortho and mono-ortho polychlorinated biphenyls (PCBs) known to be strong P450 RGS inducers were found to be at very low or non-detectable levels in samples with the highest P450 RGS responses. The profound difference in PAH profiles for the two most contaminated sitessuggested that this assay is especially sensitive for selected PAHs with greater than four rings. Combined with previous results, the P450 RGS shows promise as a useful screening tool for predicting deleterious biological effects resulting from CYP1A1-inducing, sediment-associated chemicals, particularly high molecular weight PAHs. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 21:06:05