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Titolo:
Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins andbridges two other cleavage factors
Autore:
de Vries, H; Ruegsegger, U; Hubner, W; Friedlein, A; Langen, H; Keller, W;
Indirizzi:
Univ Basel, Bioctr, Dept Cell Biol, CH-4056 Basel, Switzerland Univ BaselBasel Switzerland CH-4056 ll Biol, CH-4056 Basel, Switzerland F Hoffmann La Roche & Co Ltd, Roche Genet, CH-4070 Basel, Switzerland F Hoffmann La Roche & Co Ltd Basel Switzerland CH-4070 asel, Switzerland
Titolo Testata:
EMBO JOURNAL
fascicolo: 21, volume: 19, anno: 2000,
pagine: 5895 - 5904
SICI:
0261-4189(20001101)19:21<5895:HPCFIC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA POLYADENYLATION; TRANSCRIPTION FACTOR-IIH; FACTOR I-M; SPECIFICITY FACTOR; SPLICING FACTOR; POLY(A)-BINDING PROTEIN; MOLECULAR-CLONING; BINDING PROTEIN; POLYMERASE-II; SUBUNIT;
Keywords:
mRNA; pre-mRNA 3 ' end processing; splicing; transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Keller, W Univ Basel, Bioctr, Dept Cell Biol, Klingelbergstr 70, CH-4056 Basel, Switzerland Univ Basel Klingelbergstr 70 Basel Switzerland CH-4056 tzerland
Citazione:
H. de Vries et al., "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins andbridges two other cleavage factors", EMBO J, 19(21), 2000, pp. 5895-5904

Abstract

Six different protein factors are required in vitro for 3' end formation of mammalian pre-mRNAs by endonucleolytic cleavage and polyadenylation. Fiveof the factors have been purified and most of their components cloned, butcleavage factor IIm (CF IIm) remained uncharacterized. We have purified CFIIm from HeLa cell nuclear extract by several chromatographic steps. During purification, CF IIm activity separated into two components, one essential (CF IIA(m)) and one stimulatory (CF IIBm) for the cleavage reaction. CF IIA(m) fractions contain the human homologs of two yeast 3' end processing factors, Pcf11p and Clp1p, as well as cleavage factor I-m (CF I-m) and several splicing and transcription factors. We report the cloning of hClp1 and show that it is a genuine subunit of CF IIA(m). Antibodies directed against hClp1 deplete cleavage activity, but not polyadenylation activity from HeLacell nuclear extract. hClp1 interacts with CF I-m and the cleavage and polyadenylation specificity factor CPSF, suggesting that it bridges these two 3' end processing factors within the cleavage complex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:08:32