Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Early exclusion of Hand1-deficient cells from distinct regions of the leftventricular myocardium in chimeric mouse embryos
Autore:
Riley, PR; Gertsenstein, M; Dawson, K; Cross, JC;
Indirizzi:
Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Program Dev & Fetal Hlth, Toronto, ON M5G 1X5, Canada Mt Sinai Hosp Toronto ON Canada M5G 1X5 Hlth, Toronto, ON M5G 1X5, Canada Univ Toronto, Dept Obstet & Gynaecol, Toronto, ON M5G 1X5, Canada Univ Toronto Toronto ON Canada M5G 1X5 aecol, Toronto, ON M5G 1X5, Canada Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5G 1X5, Canada Univ Toronto Toronto ON Canada M5G 1X5 Genet, Toronto, ON M5G 1X5, Canada
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 227, anno: 2000,
pagine: 156 - 168
SICI:
0012-1606(20001101)227:1<156:EEOHCF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BHLH TRANSCRIPTION FACTOR; CARDIAC MORPHOGENESIS; HEART DEVELOPMENT; NEURAL CREST; NEUREGULIN RECEPTOR; GENE EHAND; STEM-CELLS; EXPRESSION; DEFECTS; LACKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Cross, JC Univ Calgary, Fac Med, Hlth Sci Ctr, Dept Biochem & Mol Biol,Genes & Dev Res Grp, Room 2279,3330 Hosp Dr NW, Calgary, AB T2N 4N1, Canada Univ Calgary Room 2279,3330 Hosp Dr NW Calgary AB Canada T2N 4N1
Citazione:
P.R. Riley et al., "Early exclusion of Hand1-deficient cells from distinct regions of the leftventricular myocardium in chimeric mouse embryos", DEVELOP BIO, 227(1), 2000, pp. 156-168

Abstract

The basic helix-loop-helix transcription factor gene Hand1 has been implicated in development of the heart. However, the early lethality of Hand1-null mutant mouse embryos has precluded a precise understanding of its function. In this study, we have generated Hand1 homozygous mutant ES cells and performed in vitro differentiation experiments and chimeric analysis to studythe role of Hand1 function during cardiac development. Hand1-null ES cellswere able to differentiate into beating cardiomyocytes in vitro that expressed cardiac myosin and several cardiac-specific transcripts including Nkx2-5, alpha -cardiac actin, and the myofilament genes myosin light chain 2a and 2v. In chimeras derived from Hand1-null ES cells and ROSA26 embryos, mutant cells were underrepresented in the left caudal region of the linear heart tube at E8.0. By E9.5, after cardiac looping, mutant cells were underrepresented in the anterior region of the outer curvature of the left ventricular myocardium, but did contribute to other parts of the left ventricle andto other cardiac chambers. These results imply that Hand1 is not essentialfor differentiation of ventricular cardiomyocytes. Hand1-null cells were also underrepresented in several other regions of later embryos, including the rhombencephalic neural tube that was associated with a deficiency of mutant cells in the neural crest cell-derived cardiac outflow tract and first branchial arch. In summary, Hand1 has cell-autonomous functions during cardiac morphogenesis in both mesodermal and neural crest derivatives. (C) 2000Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:21:40