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Titolo:
Internalization and half-life of membrane-bound macrophage colony-stimulating factor
Autore:
Tang, SS; Liu, HZ; Chen, GB; Rao, Q; Geng, YQ; Zheng, GG; Zheng, DX; Wu, KF;
Indirizzi:
Chinese Acad Med Sci, State Key Lab Expt Hematol, Inst Hematol, Tianjin 300020, Peoples R China Chinese Acad Med Sci Tianjin Peoples R China 300020020, Peoples R China Peking Union Med Coll, Tianjin 300020, Peoples R China Peking Union Med Coll Tianjin Peoples R China 300020 20, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 18, volume: 45, anno: 2000,
pagine: 1697 - 1703
SICI:
1001-6538(200009)45:18<1697:IAHOMM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-I; RECEPTOR; ADHESION; CELLS;
Keywords:
macrophage colony-stimulating factor; endocytosis; half-life;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, KF Chinese Acad Med Sci, State Key Lab Expt Hematol, Inst Hematol, Tianjin 300020, Peoples R China Chinese Acad Med Sci Tianjin Peoples R China300020 oples R China
Citazione:
S.S. Tang et al., "Internalization and half-life of membrane-bound macrophage colony-stimulating factor", CHIN SCI B, 45(18), 2000, pp. 1697-1703

Abstract

The binding of recombinant human macrophage colony-stimulating factor (M-CSF) soluble receptor (rh-M-CSF-sR) to membrane-bound macrophage colony stimulating factor (m-M-CSF) and the internalization and recycling of rh-M-CSF-sR/m-M-CSF complexes mediated by m-M-CSF were studied with a model of J6-1 cell line. The results indicated that m-M-CSF bound rh-M-CSF-sR with high affinity (k(d) = 1.78 x 10(-12) mol/L) and mediated a temperature- and energy-dependent internalization of rh-M-CSF-sR, and that internalized rh-M-CSF-sR could return to ii-to cell surface in an m-M-CSF-bound state, suggestingthat m-M-CSF may have a capability to mediate the internalization and recycling of rh-M-CSF-sR/m-M-CSF complexes. In addition, the half-lives of cell-associated M-CSF and its receptor of stimulated normal human cord blood mononuclear cells (CBMCs) and 4 leukemic cell lines were measured by indirectimmunofluorescence and flow cytometry. The results showed that the half-lives of the various kinds of WI-CSF isoforms and M-CSF-R in 4 leukemic cells, except those of membrane-bound M-CSF receptor (M-CSF-mR) of K562 and HL-60 cells, were longer than those of corresponding M-CSF isoform and its receptor in stimulated normal human CBMCs, suggesting that there is a slow degradation rate of cell-associated M-CSF and its receptor in leukemic cells.

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Documento generato il 14/07/20 alle ore 06:18:01