Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of novel cytochrome P450 1A genes from five marine mammal species
Autore:
Teramitsu, I; Yamamoto, Y; Chiba, I; Iwata, H; Tanabe, S; Fujise, Y; Kazusaka, A; Akahori, F; Fujita, S;
Indirizzi:
Hokkaido Univ, Grad Sch Vet Med, Dept Environm Vet Sci, Toxicol Lab,Kita Ku, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan Ehime Univ, Dept Environm Conservat, Matsuyama, Ehime 7908566, Japan EhimeUniv Matsuyama Ehime Japan 7908566 Matsuyama, Ehime 7908566, Japan Inst Cetacean Res, Chuo Ku, Tokyo 1040055, Japan Inst Cetacean Res TokyoJapan 1040055 Res, Chuo Ku, Tokyo 1040055, Japan Azabu Univ, Sch Vet Med, Dept Environm Hlth, Kanagawa 2298501, Japan AzabuUniv Kanagawa Japan 2298501 Environm Hlth, Kanagawa 2298501, Japan
Titolo Testata:
AQUATIC TOXICOLOGY
fascicolo: 2, volume: 51, anno: 2000,
pagine: 145 - 153
SICI:
0166-445X(200012)51:2<145:IONCP1>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEALS PHOCA-VITULINA; FUNDULUS-HETEROCLITUS; DELPHINAPTERUS-LEUCAS; PHYLOGENETIC ANALYSIS; RAT-LIVER; SEQUENCE; CDNA; PCB; ORGANOCHLORINES; ACCUMULATION;
Keywords:
marine mammals; cytochrome P-450 1A; degenerate polymerase chain reaction; phylogenetic analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Fujita, S Hokkaido Univ, Grad Sch Vet Med, Dept Environm Vet Sci, Toxicol Lab,Kita Ku, N18,W9, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ N18,W9 Sapporo Hokkaido Japan 0600818 00818, Japan
Citazione:
I. Teramitsu et al., "Identification of novel cytochrome P450 1A genes from five marine mammal species", AQUAT TOX, 51(2), 2000, pp. 145-153

Abstract

Marine mammals, being endangered by the chronic exposure of hydrophobic environmental contaminants as an asserting result of global pollution, are especially focused as indicators for organochlorine pollution. The use of contaminant-induced xenobiotic metabolizers, particularly P450 (CYP) 1A, in marine mammals can be effective as potential biomarkers of the contaminant exposure and/or toxic effects. In this study, we identified the first marine mammalian CYPs. Six novel CYP1A cDNA fragments were cloned from the livers of marine mammal species, minke whale (Balaenoptera acutorostrata), dall's porpoise (Phocoenoides dalli), steller sea lion (Eumetopias jubatus), largha seal (Phoca largha), and ribbon seal (Phoca fasciata) by the method of reverse transcription/polymerase chain reaction (RT/PCR); two distinct fragments were from steller sea lion and one fragment each was obtained from the other species. Five of the fragments, one from each species, were classified in the subfamily of CYP1A1, and the other fragment cloned from steller sea lion was designated CYP1A2. Degenerate PCR primers were used to amplify the fragments from liver cDNAs. The deduced amino acid sequences of these fragment CYP1As showed identities ranging from 50.0 to 94.3% with other knownvertebrate CYPs in the subfamily of CYP1A, including those from fish, chicken, and terrestrial mammals. The isolated fragments were used to constructa molecular phylogeny, along with other vertebrate CYP1A cDNAs cut down insize to the corresponding region of 265 bp in which those newly determinedfragments were cloned. This phylogenetic analysis by the maximum parsimonymethod using the PHYLIP program suggests two distinct evolutional pathwaysfor aquatic mammalian CYP1As, compatible to a conservative taxonomy. Pinniped genes are clustered together with dog gene, forming a carnivore group, and cetaceans form another branch. Identification of CYP1A genes in marine mammals will be an introductory step to provide new insights into the metabolic or toxicological functions of CYP1As in these animals. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:41:05