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Titolo:
Inducible nitric oxide synthase - Knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan
Autore:
Cuzzocrea, S; Mazzon, E; Calabro, G; Dugo, L; De Sarro, A; van de Loo, FAJ; Caputi, AP;
Indirizzi:
Univ Messina, Policlin Univ, Sch Med, Inst Pharmacol, I-98123 Messina, Italy Univ Messina Messina Italy I-98123 nst Pharmacol, I-98123 Messina, Italy Univ Messina, Sch Med, Dept Biomorphol, I-98123 Messina, Italy Univ Messina Messina Italy I-98123 pt Biomorphol, I-98123 Messina, Italy Univ Nijmegen Hosp, Dept Rheumatol, NL-6500 HB Nijmegen, Netherlands Univ Nijmegen Hosp Nijmegen Netherlands NL-6500 HB Nijmegen, Netherlands
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
fascicolo: 5, volume: 162, anno: 2000,
pagine: 1859 - 1866
SICI:
1073-449X(200011)162:5<1859:INOS-K>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSE SYNTHETASE; PEROXYNITRITE-MEDIATED OXIDATION; CELLULAR-ENERGY DEPLETION; INDUCED INFLAMMATION; TYROSINE NITRATION; LOCAL INFLAMMATION; INDUCED MODELS; DNA-DAMAGE; RAT; INHIBITOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Cuzzocrea, S Univ Messina, Policlin Univ, Sch Med, Inst Pharmacol, Via C Valeria, I-98123 Messina, Italy Univ Messina Via C Valeria Messina Italy I-98123 sina, Italy
Citazione:
S. Cuzzocrea et al., "Inducible nitric oxide synthase - Knockout mice exhibit resistance to pleurisy and lung injury caused by carrageenan", AM J R CRIT, 162(5), 2000, pp. 1859-1866

Abstract

In the present study, we investigated the role of inducible (or type 2) nitric oxide synthase (iNOS) in the development of acute inflammation by comparing the responses in wild-type mice (WT) and mice lacking (knockout [KO]). When compared with carrageenan-treated iNOS-WT mice, iNOS-KO mice that had received carrageenan exhibited a reduced degree of pleural exudation and polymorphonuclear cell migration. Lung myeloperoxidase (MPO) activity and lipid peroxidation were significantly reduced in iNOS-KO mice in comparison with iNOSWT mice. Immunohistochemical analysis for nitrotyrosine revealed positive staining in lungs from carrageenan-treated iNOS-WT mice. Lung tissue sections from carrageenan-treated iNOS-WT mice showed positive staining for poly adenosine diphosphate (ADP)-ribose synthetase that was mainly localized in alveolar macrophages and in airway epithelial cells. The intensity and degree of staining for nitrotyrosine and poly-ADP-ribose synthetase were markedly reduced in tissue sections from carrageenan-treated iNOS-KO mice. The inflamed lungs of iNOS-KO mice also showed an improved histologic status. Furthermore, a significant reduction in the suppression of energy status, in DNA strand breakage, and in decreased cellular levels of nicotinamide adenine dinucleotide (NAD(+)) was observed ex vivo in macrophages harvested from the pleural cavity of iNOS-KO mice subjected to carrageenan-inducedpleurisy. Taken together, our results clearly show that iNOS plays an important role in the acute inflammatory response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:03:18