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Titolo:
Determination of fluoxetine and its metabolite norfluoxetine in human liver microsomes by reversed-phase HPLC in vitro
Autore:
Liu, ZQ; Cheng, ZN; Wang, W; Tan, ZR; Ou-Yang, DS; Zhou, HH;
Indirizzi:
Hunan Med Univ, Pharmacogenet Res Inst, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 a 410078, Peoples R China
Titolo Testata:
ACTA PHARMACOLOGICA SINICA
fascicolo: 11, volume: 21, anno: 2000,
pagine: 1027 - 1030
SICI:
0253-9756(200011)21:11<1027:DOFAIM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; FLUORESCENCE DETECTION; ULTRAVIOLET DETECTION; GAS-CHROMATOGRAPHY; N-DEMETHYLATION; HUMAN PLASMA; IN-VITRO;
Keywords:
fluoxetine; high pressure liquid chromatography; liver microsomes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Zhou, HH Hunan Med Univ, Pharmacogenet Res Inst, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 Peoples R China
Citazione:
Z.Q. Liu et al., "Determination of fluoxetine and its metabolite norfluoxetine in human liver microsomes by reversed-phase HPLC in vitro", ACT PHAR SI, 21(11), 2000, pp. 1027-1030

Abstract

AIM: A high-performance liquid chromatography (HPLC) method was developed for the determination of fluoxetine (FLU) and its metabolite norfluoxetine (N-FLU) in human liver microsomes in vitro. METHODS: An incubation buffer containing human liver microsomes, NADPH-generating system, and FLU, after termination of enzyme reaction and addition of nortriptyline (NOR) as internal standard (IS), was extracted with n-hexane/acetonitrile, and separated on a reversed-phase ODS column. Detection was achieved at 226 nm by ultraviolet detector (UV). RESULTS: The limit of detection was 5 mug/L for both FLUand N-FLU. No potential interference was found. The method provides recoveries of up to 94 % - 104 % and acceptable coefficients of variation were found for both within-run ( < 7.8 %) and day to day (< 9.1 %) assays. CONCLUSION: This method is rapid, sensitive, and simple for studying the metabolism of FLU and N-FLU.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 23:05:57