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Titolo:
The use of yeast two-hybrid screens in studies of protein : protein interactions involved in trafficking
Autore:
Stephens, DJ; Banting, G;
Indirizzi:
Univ Bristol, Dept Biochem, Bristol BS8 1TH, Avon, England Univ Bristol Bristol Avon England BS8 1TH Bristol BS8 1TH, Avon, England EMBL, Dept Cell Biol & Cell Biophys, Heidelberg, Germany EMBL HeidelbergGermany t Cell Biol & Cell Biophys, Heidelberg, Germany
Titolo Testata:
TRAFFIC
fascicolo: 10, volume: 1, anno: 2000,
pagine: 763 - 768
SICI:
1398-9219(200010)1:10<763:TUOYTS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOMAIN-CONTAINING PROTEIN; ADAPTER MEDIUM CHAINS; SORTING SIGNALS; SUBUNIT INTERACTIONS; BINDING-PROTEIN; EPS15 HOMOLOGY; COMPLEX; DYNAMIN; ENDOCYTOSIS; TGN38;
Keywords:
trafficking; two-hybrid; vesicular transport;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Banting, G Univ Bristol, Dept Biochem, Bristol BS8 1TH, Avon, England UnivBristol Bristol Avon England BS8 1TH 8 1TH, Avon, England
Citazione:
D.J. Stephens e G. Banting, "The use of yeast two-hybrid screens in studies of protein : protein interactions involved in trafficking", TRAFFIC, 1(10), 2000, pp. 763-768

Abstract

The yeast two-hybrid system has provided a convenient means to both screenfor proteins that interact with a protein of interest and to characterise the known interaction between two proteins. Several groups with an interestin the molecular mechanisms that underlie discrete steps along traffickingpathways have exploited the yeast two-hybrid system. Here, we provide a brief background to the technology, attempt to point out some of the pitfallsand benefits of the different systems that can be employed, and mention some of the areas (within the trafficking field) where yeast two-hybrid interaction assays have been particularly informative.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:05:21