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Titolo:
Increased in vitro binding and fertilizing ability of mouse sperm exposed to a synthetic peptide
Autore:
Magargee, SF; Cramer, PG; Hammerstedt, RH;
Indirizzi:
Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA PennState Univ University Pk PA USA 16802 l, University Pk, PA 16802 USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 4, volume: 57, anno: 2000,
pagine: 406 - 411
SICI:
1040-452X(200012)57:4<406:IIVBAF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; BULL SPERM; CRYOPRESERVATION; ASSAY; SPERMATOZOA;
Keywords:
mouse sperm; IVF; fertilization; synthetic peptide; prosaposin; sperm-ova binding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Hammerstedt, RH BioPore Inc, POB 10074, State Coll, PA 16805 USA BioPore Inc POB 10074 State Coll PA USA 16805 PA 16805 USA
Citazione:
S.F. Magargee et al., "Increased in vitro binding and fertilizing ability of mouse sperm exposed to a synthetic peptide", MOL REPROD, 57(4), 2000, pp. 406-411

Abstract

We report use of an in vitro assay (Barbato et at., 1998: Biol Reprod 58:686-699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999:J Androl 20: 42-46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from C57B1/6, CD1, and CF1 mice (4 replicates each) were evaluated for binding ability after exposure to 0 (control) and 80-1280 pM peptide. For control sperm, endogenous binding was C57Bl/ 6<CD1= CF1 (P<0.05,1-way ANOVA). Across all three strains, exposure to >320 pM peptide increased relative binding of sperm (P<0.05; 2-way general linear model; GLM). Strains differed both in basal binding ability and in response to synthetic peptide. To determine if IVF rate increased after exposure of sperm to peptide, ova from B6C3 mice (four replicate pools) were collected after eCG and hCG stimulation. Cumulus-oocyte complexes (COC; 8-15 ova in each of 3-6 drops/treatment) were incubated with hyperactivated C57B1/6 sperm at <similar to>1500 sperm per ovum. Data for incubations were corrected for false-positive classification to yield a better estimate of true cleavage rate, and then related to results observed with a tenfold greater sperm concentration. Relative cleavage rates were 0 peptide (0.48); 420 pM (0.78, P<0.05); and 840 pM (0.90, P<0.01; GLM and Tukey tests). IVF rate was increased by exposure of mouse sperm to peptide at concentrations effective in the in vitro assay, and use of peptide allowed use of 1/10 as many sperm. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:03:00