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Titolo:
Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: Identification of a c-Src/Cav-1/Grb7signaling cassette
Autore:
Lee, H; Volonte, D; Galbiati, F; Iyengar, P; Lublin, DM; Bregman, DB; Wilson, MT; Campos-Gonzalez, R; Bouzahzah, B; Pestell, RG; Scherer, PE; Lisanti, MP;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx, NY 10461USA Yeshiva Univ Albert Einstein Coll Med, Albert Einstein Canc Ctr, Bronx, NY10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx, NY10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USAYeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Dev & Mol Biol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Pathol, St Louis, MO 63110 USA BD Transduct Labs, Lexington, KY 40511 USA BD Transduct Labs Lexington KYUSA 40511 ct Labs, Lexington, KY 40511 USA
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 11, volume: 14, anno: 2000,
pagine: 1750 - 1775
SICI:
0888-8809(200011)14:11<1750:CAGFPO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUS-SARCOMA VIRUS; PROTEIN-TYROSINE KINASES; RICH MEMBRANE DOMAINS; HUMAN BREAST-CANCER; ONCOGENICALLY TRANSFORMED-CELLS; TRANS-GOLGI-NETWORK; D7S522 LOCUS 7Q31.1; SMOOTH-MUSCLE CELLS; PLASMA-MEMBRANE; IN-VIVO;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
103
Recensione:
Indirizzi per estratti:
Indirizzo: Lisanti, MP Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, 1300 Morris Pk Ave,Room 202 Golding Bldg, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med 1300 Morris Pk Ave,Room 202 Golding Bldg Bronx NY USA10461
Citazione:
H. Lee et al., "Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: Identification of a c-Src/Cav-1/Grb7signaling cassette", MOL ENDOCR, 14(11), 2000, pp. 1750-1775

Abstract

Caveolin-1 was first identified as a phosphoprotein in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. Tyrosine 14 is now thought tobe the principal site for recognition by c-Src kinase; however, little is known about this phosphorylation event. Here, we generated a monoclonal antibody (mAb) probe that recognizes only tyrosine 14-phosphorylated caveolin-1. Using this approach, we show that caveolin-1 (Y14) is a specific tyrosine kinase substrate that is constitutively phosphorylated in Src- and Abl-transformed cells and transiently phosphorylated in a regulated fashion during growth factor signaling. We also provide evidence that tyrosine-phosphorylated caveolin-1 is localized at the major sites of tyrosine-kinase signaling, i.e. focal adhesions. By analogy with other signaling events, we hypothesized that caveolin-1 could serve as a docking site for pTyr-binding molecules. In support of this hypothesis, we show that phosphorylation of caveolin-1 on tyrosine 14 confers binding to Grb7 (an SH2-domain containing protein) both in vitro and in vivo. Furthermore, we demonstrate that binding of Grb7 to tyrosine 14-phosphorylated caveolin-1 functionally augments anchorage-independent growth and epidermal growth factor (EGF)-stimulated cell migration. We discuss the possible implications of our findings in the contextof signal transduction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 11:05:17