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Titolo:
Studies on substances contained in spices inducing allergic reaction
Autore:
Tsuda, Y; Kataoka, H; Semma, M; Ito, Y;
Indirizzi:
Mukogawa Womens Univ, Fac Pharmaceut Sci, Nishinomiya, Hyogo 6638179, Japan Mukogawa Womens Univ Nishinomiya Hyogo Japan 6638179 Hyogo 6638179, Japan
Titolo Testata:
JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN
fascicolo: 5, volume: 41, anno: 2000,
pagine: 307 - 311
SICI:
0015-6426(200010)41:5<307:SOSCIS>2.0.ZU;2-E
Fonte:
ISI
Lingua:
JPN
Soggetto:
AW METHOD; INDUCTION;
Keywords:
spices; Rosmarinus officinalis L.; allergy; abdominal wall method; sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuda, Y Mukogawa Womens Univ, Fac Pharmaceut Sci, 11-68 Koshien Kyuban Cho, Nishinomiya, Hyogo 6638179, Japan Mukogawa Womens Univ 11-68 Koshien Kyuban Cho Nishinomiya Hyogo Japan 6638179
Citazione:
Y. Tsuda et al., "Studies on substances contained in spices inducing allergic reaction", J FOOD HYG, 41(5), 2000, pp. 307-311

Abstract

We studied the allergenic potential of fifteen kinds of spices in mice using the AW method as an experimental system. Rosemary, caraway, sweet basil,garlic, estragon and mace were found to induce significant anaphylactic reactions in this system. Previously, we reported that rosemary exhibited significant inhibition of allergic reaction in mice. Therefore, the rosemary extract was fractionatedwith an ultrafiltration membrane (10 kDa cut-off) in order to investigate the major component of rosemary affecting allergic reaction. While the high-molecular-weight fraction induced anaphylactic reaction, the low-molecular-weight fraction showed significant inhibition of allergic reaction. After separation of the rosemary extract by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), western-blot analysis indicated that two proteins were able to bind to IgE of the serum of mouse sensitized with rosemary extract. Furthermore, mice sensitized with even the SDS-PAGE gel containing the protein band (26 similar to 27 kDa) were shown to exhibit a significant anaphylactic reaction by the AW method.

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Documento generato il 10/07/20 alle ore 09:02:47