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Titolo:
Subcellular distribution of envoplakin and periplakin: Insights into theirrole as precursors of the epidermal cornified envelope
Autore:
DiColandrea, T; Karashima, T; Maatta, A; Watt, FM;
Indirizzi:
Imperial Canc Res Fund, Keratinocyte Lab, London WC2A 3PX, England Imperial Canc Res Fund London England WC2A 3PX London WC2A 3PX, England
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 3, volume: 151, anno: 2000,
pagine: 573 - 585
SICI:
0021-9525(20001030)151:3<573:SDOEAP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BULLOUS PEMPHIGOID ANTIGEN; KERATIN INTERMEDIATE FILAMENTS; CULTURED-CELLS; TERMINAL DIFFERENTIATION; STRUCTURAL-ANALYSIS; PLAKIN FAMILY; COILED-COIL; PLECTIN; DESMOPLAKIN; PROTEINS;
Keywords:
envoplakin; periplakin; desmosomes; cornified envelope; keratinocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Watt, FM Imperial Canc Res Fund, Keratinocyte Lab, POB 123,44 Lincolns InnFields, London WC2A 3PX, England Imperial Canc Res Fund POB 123,44 Lincolns Inn Fields London England WC2A 3PX
Citazione:
T. DiColandrea et al., "Subcellular distribution of envoplakin and periplakin: Insights into theirrole as precursors of the epidermal cornified envelope", J CELL BIOL, 151(3), 2000, pp. 573-585

Abstract

Envoplakin and periplakin are two plakins that are precursors of the epidermal cornified envelope, We studied their distribution and interactions by transfection of primary human keratinocytes and other cells. Full-length periplakin localized to desmosomes, the interdesmosomal plasma membrane and intermediate filaments. Full length envoplakin also localized to desmosomes,but mainly accumulated in nuclear and cytoplasmic aggregates with associated intermediate filaments. The envoplakin rod domain was required for aggregation and the periplakin rod domain was necessary and sufficient to redistribute envoplakin to desmosomes and the cytoskeleton, confirming earlier predictions that the proteins can heterodimerize, The linker domain of each protein was required for intermediate filament association. Like the NH2 terminus of desmoplakin, that of periplakin localized to desmosomes; however, in addition, the periplakin NH2 terminus accumulated at cell surface microvilli in association with cortical actin, Endogenous periplakin was redistributed from microvilli when keratinocytes were treated with the actin disrupting drug Latrunculin B. We propose that whereas envoplakin and periplakin can localize independently to desmosomes, the distribution of envoplakin atthe interdesmosomal plasma membrane depends on heterodimerization with periplakin and that the NH2 terminus of periplakin therefore plays a key role in forming the scaffold on which the cornified envelope is assembled.

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Documento generato il 25/09/20 alle ore 00:36:25